Enhancing the culturability of bacteria from the gastrointestinal tract of farmed adult turbot Scophthalmus maximus
文献类型：期刊论文
| 作者 | Xing Mengxin1,2; Hou Zhanhui1; Qu Yanmei1; Liu Bin1 |
| 刊名 | CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY
 |
| 出版日期 | 2014-03-01 |
| 卷号 | 32期号:2页码:316-325 |
| 关键词 | turbot gastrointestinal supernatant 16S rRNA gene operational taxonomic units (OTUs) cultivation efficiency |
| 通讯作者 | Liu, B (reprint author), Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China. |
| 英文摘要 | Eighteen agar media were tested for the culture of gut-associated bacteria from farmed adult turbot (Scophthalmus maximus), including 16 agar media with or without 1% gastrointestinal (GI) supernatant, or with 2% or 4% GI supernatant. A total of 1 711 colonies were analyzed and 24 operational taxonomic units (OTUs) were identified. The greatest bacterial diversity was isolated on Zobell 2216E/Zobell 2216E+ agar media, whereas MRS/MRS+ agar media produced a low diversity of colonies. Agar media with GI supernatant (1%, 2%, or 4%) showed increased diversity and yielded different profiles of OTUs from the corresponding original media, suggesting that GI supernatant provides substances that enhance the culture efficiency of bacteria from the turbot GI tract. The large majority of the colonies (82%) were gamma-Proteobacteria, whereas 15.6% and 2.4% of colonies were Firmicutes and Actinobacteria, respectively. At the genus level, 49.4% of all colonies were assigned to Vibrio. Other potential pathogens, including Pseudomonas, Photobacterium, and Enterobacter, and potential probiotics, including Bacillus, Paenibacillus, and Pseudomonas, were also isolated on agar media. Most cultured bacteria belonged to species that were first described in the turbot GI tract. The impact of these species on turbot physiology and health should be investigated further. |
| 学科主题 | Marine & Freshwater Biology; Oceanography |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000332739600007 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/24110]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Xing Mengxin,Hou Zhanhui,Qu Yanmei,et al. Enhancing the culturability of bacteria from the gastrointestinal tract of farmed adult turbot Scophthalmus maximus[J]. CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,2014,32(2):316-325. |
| APA | Xing Mengxin,Hou Zhanhui,Qu Yanmei,&Liu Bin.(2014).Enhancing the culturability of bacteria from the gastrointestinal tract of farmed adult turbot Scophthalmus maximus.CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,32(2),316-325. |
| MLA | Xing Mengxin,et al."Enhancing the culturability of bacteria from the gastrointestinal tract of farmed adult turbot Scophthalmus maximus".CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY 32.2(2014):316-325. |
入库方式： OAI收割
来源：海洋研究所
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