Exploring valid internal-control genes in Porphyra yezoensis (Bangiaceae) during stress response conditions
文献类型：期刊论文
| 作者 | Wang Wenlei1; Wu Xiaojie1; Wang Chao2; Jia Zhaojun1; He Linwen2; Wei Yifan2; Niu Jianfeng2,3; Wang Guangce1,2 |
| 刊名 | CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY
 |
| 出版日期 | 2014-07-01 |
| 卷号 | 32期号:4页码:783-791 |
| 关键词 | constitutive expression gene housekeeping gene Porphyra yezoensis Ueda real-time quantitative PCR stress responding |
| 通讯作者 | Niu, JF (reprint author), Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China. |
| 英文摘要 | To screen the stable expression genes related to the stress (strong light, dehydration and temperature shock) we applied Absolute real-time PCR technology to determine the transcription numbers of the selected test genes in Porphyra yezoensis, which has been regarded as a potential model species responding the stress conditions in the intertidal. Absolute real-time PCR technology was applied to determine the transcription numbers of the selected test genes in Porphyra yezoensis, which has been regarded as a potential model species in stress responding. According to the results of photosynthesis parameters, we observed that Y(II) and F-v/F-m were significantly affected when stress was imposed on the thalli of Porphyra yezoensis, but underwent almost completely recovered under normal conditions, which were collected for the following experiments. Then three samples, which were treated with different grade stresses combined with salinity, irradiation and temperature, were collected. The transcription numbers of seven constitutive expression genes in above samples were determined after RNA extraction and cDNA synthesis. Finally, a general insight into the selection of internal control genes during stress response was obtained. We found that there were no obvious effects in terms of salinity stress (at salinity 90) on transcription of most genes used in the study. The 18S ribosomal RNA gene had the highest expression level, varying remarkably among different tested groups. RPS8 expression showed a high irregular variance between samples. GAPDH presented comparatively stable expression and could thus be selected as the internal control. EF-1 alpha showed stable expression during the series of multiple-stress tests. Our research provided available references for the selection of internal control genes for transcripts determination of P. yezoensis. |
| 学科主题 | Marine & Freshwater Biology; Oceanography |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000339046200006 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/24112]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 作者单位 | 1.Tianjin Univ Sci & Technol, Coll Marine Sci & Engn, Tianjin 300457, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 3.Chinese Acad Sci, Nantong Branch, Inst Oceanol, Nantong 226006, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang Wenlei,Wu Xiaojie,Wang Chao,et al. Exploring valid internal-control genes in Porphyra yezoensis (Bangiaceae) during stress response conditions[J]. CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,2014,32(4):783-791. |
| APA | Wang Wenlei.,Wu Xiaojie.,Wang Chao.,Jia Zhaojun.,He Linwen.,...&Wang Guangce.(2014).Exploring valid internal-control genes in Porphyra yezoensis (Bangiaceae) during stress response conditions.CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,32(4),783-791. |
| MLA | Wang Wenlei,et al."Exploring valid internal-control genes in Porphyra yezoensis (Bangiaceae) during stress response conditions".CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY 32.4(2014):783-791. |
入库方式： OAI收割
来源：海洋研究所
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