HdhCTL1 is a novel C-type lectin of abalone Haliotis discus hannai that agglutinates Gram-negative bacterial pathogens
文献类型：期刊论文
| 作者 | Zhang, Jian1,3; Qiu, Reng1,2; Hu, Yong-hua1 |
| 刊名 | FISH & SHELLFISH IMMUNOLOGY
 |
| 出版日期 | 2014-12-01 |
| 卷号 | 41期号:2页码:466-472 |
| 关键词 | Haliotis discus hannai C-type lectin Agglutination |
| 通讯作者 | Hu, YH (reprint author), Chinese Acad Sci, Inst Oceanol, 7 Nanhai Rd, Qingdao 266071, Peoples R China. |
| 英文摘要 | C-type lectins (CTLs) are Ca2+-dependent carbohydrate recognition proteins, which play important roles in the innate immunity of both vertebrates and invertebrates. In this study, we identified and characterized a C-type lectin (named HdhCTL1) from Pacific abalone, Haliotis discus hannai. HdhCTL1 is composed of 176 amino acid residues and shares low (23.9%) identity with the known CTL of abalone. HdhCTL1 possesses a putative signal peptide and a carbohydrate-recognition domain (CRD) typical of CTLs. The CRD of HdhCTL1 contains four disulfide bond-forming cysteine residues that are highly conserved in CTLs. HdhCTL1 mRNA was detected in a wide range of tissues and expressed abundantly in the digestive gland.,Experimental infection with the bacterial pathogen Vibrio anguillarum significantly upregulated HdhCTL1 expression in a time-dependent manner. Recombinant HdhCTL1 (rHdhCTL1) purified from Escherichia coli was able to agglutinate Gram-negative bacterial pathogens. The agglutinating ability of rHdhCTL1 was abolished in the presence of mannose. These results suggest that HdhCTL1 is a novel CTL which is likely to be involved in host defense against bacterial infection. (C) 2014 Elsevier Ltd. All rights reserved. |
| 学科主题 | Fisheries; Immunology; Marine & Freshwater Biology; Veterinary Sciences |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000347017500042 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/24119]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 2.Nanyang Normal Univ, China UK NYNU RRes Joint Lab Insect Biol, Nanyang 473061, Henan, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Jian,Qiu, Reng,Hu, Yong-hua. HdhCTL1 is a novel C-type lectin of abalone Haliotis discus hannai that agglutinates Gram-negative bacterial pathogens[J]. FISH & SHELLFISH IMMUNOLOGY,2014,41(2):466-472. |
| APA | Zhang, Jian,Qiu, Reng,&Hu, Yong-hua.(2014).HdhCTL1 is a novel C-type lectin of abalone Haliotis discus hannai that agglutinates Gram-negative bacterial pathogens.FISH & SHELLFISH IMMUNOLOGY,41(2),466-472. |
| MLA | Zhang, Jian,et al."HdhCTL1 is a novel C-type lectin of abalone Haliotis discus hannai that agglutinates Gram-negative bacterial pathogens".FISH & SHELLFISH IMMUNOLOGY 41.2(2014):466-472. |
入库方式： OAI收割
来源：海洋研究所
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