Rock bream (Oplegnathus fasciatus) IL-12p40: Identification, expression, and effect on bacterial infection
文献类型：期刊论文
| 作者 | Zhang, Lu1,2; Zhang, Bao-cun1,2; Hu, Yong-hua1 |
| 刊名 | FISH & SHELLFISH IMMUNOLOGY
 |
| 出版日期 | 2014-08-01 |
| 卷号 | 39期号:2页码:312-320 |
| 关键词 | IL-12p40 Oplegnathus fasciatus Cytokine Immune defense Bacterial infection |
| 通讯作者 | Hu, YH (reprint author), Chinese Acad Sci, Inst Oceanol, 7 Nanhai Rd, Qingdao 266071, Peoples R China. |
| 英文摘要 | IL-12p40, also called IL-12 beta, is a subunit of the proinflammatory cytokines interleukin (IL)-12 and IL-23. In teleost, IL-12p40 homologues have been identified in several species, however, the biological function of fish IL-12p40 is essentially unknown. In this work, we reported the identification and analysis of an IL-12p40, OflL-12p40, from rock bream (Oplegnathus fasciatus). OflL-12p40 is composed of 361 amino acids and possesses a conserved IL-12p40 domain and a WSxWS signature motif characteristic of known IL-12p40. Constitutive expression of OflL-12p40 occurred in multiple tissues and was highest in kidney. Experimental infection with bacterial pathogen upregulated the expression of OfIL-12p40 in kidney and spleen in a time-dependent manner. Purified recombinant OflL-12p40 (rOflL-12p40) stimulated the respiratory burst activity of peripheral blood leukocytes in a dose-dependent manner. rOflL-12p40 also enhanced the resistance of rock bream against bacterial infection and upregulated the expression of innate immune genes in kidney. Taken together, these results indicate that OflL-12p40 possesses cytokine-like property and plays a role in immune defense against bacterial infection. (C) 2014 Elsevier Ltd. All rights reserved. |
| 学科主题 | Fisheries; Immunology; Marine & Freshwater Biology; Veterinary Sciences |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000339773000025 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/24159]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Lu,Zhang, Bao-cun,Hu, Yong-hua. Rock bream (Oplegnathus fasciatus) IL-12p40: Identification, expression, and effect on bacterial infection[J]. FISH & SHELLFISH IMMUNOLOGY,2014,39(2):312-320. |
| APA | Zhang, Lu,Zhang, Bao-cun,&Hu, Yong-hua.(2014).Rock bream (Oplegnathus fasciatus) IL-12p40: Identification, expression, and effect on bacterial infection.FISH & SHELLFISH IMMUNOLOGY,39(2),312-320. |
| MLA | Zhang, Lu,et al."Rock bream (Oplegnathus fasciatus) IL-12p40: Identification, expression, and effect on bacterial infection".FISH & SHELLFISH IMMUNOLOGY 39.2(2014):312-320. |
入库方式： OAI收割
来源：海洋研究所
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