The involvement of cysteine-rich intestinal protein in early development and innate immunity of Asiatic hard clam, Meretrix meretrix
文献类型：期刊论文
| 作者 | Chen, Hongjian1; Yang, Xue1; Tang, Ting1; Li, Juan1; Liu, Baozhong2 ; Liu, Fengsong1; Xie, Song1,3
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| 刊名 | FISH & SHELLFISH IMMUNOLOGY
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| 出版日期 | 2014-10-01 |
| 卷号 | 40期号:2页码:435-440 |
| 关键词 | Cysteine-rich intestinal protein Meretrix meretrix Early development Immune response |
| 通讯作者 | Liu, FS (reprint author), Hebei Univ, Coll Life Sci, Baoding 071002, Peoples R China. |
| 英文摘要 | Cysteine-rich intestinal protein (CRIP), a Zn2+-binding protein, contains a single copy of the highly conserved double-zinc-finger structure known as the LIM (lin-11-isl-1-mec-3) motif. In this paper, a cDNA encoding MmCRIP was isolated from the Asiatic hard clam Meretrix meretrix. The full-length cDNA of MmCRIP consists of a 237-bp open reading frame that encodes a polypeptide of 78 amino acids with a predicted molecular weight (MW) of 8635.8 Da and theoretical isoelectric point (pI) of 9.01. Bioinformatics analysis showed that it belonged to a new member of the CRIP subfamily. Relationship analysis revealed that MmCRIP has high-levels of sequence similarity to many CRIPs reported in other animals, particularly in invertebrates. Real-time PCR analysis showed that the highest level of MmCRIP expression was in hemocyte tissue and at pediveligers stage. To investigate immune function, mature clams were challenged with Aeromonas hydrophila. During A. hydrophila infection, up-regulation of MmCRIP transcript in clam's hemocyte, gill and hepatopancreas was detected. DsRNAi (double-strand RNA interference) approach was employed to study the function of MmCRIP and the data showed that inactivation of the MmCRIP gene blocked larvae development and caused mass mortalities. The probable roles of MmCRIP in clam early development and innate immunity are presented for the first time. (C) 2014 Elsevier Ltd. All rights reserved. |
| 学科主题 | Fisheries; Immunology; Marine & Freshwater Biology; Veterinary Sciences |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000343381100011 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/24181]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 作者单位 | 1.Hebei Univ, Coll Life Sci, Baoding 071002, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 3.Univ Wisconsin, Coll Letters & Sci, Madison, WI 53706 USA |
| 推荐引用方式 GB/T 7714 | Chen, Hongjian,Yang, Xue,Tang, Ting,et al. The involvement of cysteine-rich intestinal protein in early development and innate immunity of Asiatic hard clam, Meretrix meretrix[J]. FISH & SHELLFISH IMMUNOLOGY,2014,40(2):435-440. |
| APA | Chen, Hongjian.,Yang, Xue.,Tang, Ting.,Li, Juan.,Liu, Baozhong.,...&Xie, Song.(2014).The involvement of cysteine-rich intestinal protein in early development and innate immunity of Asiatic hard clam, Meretrix meretrix.FISH & SHELLFISH IMMUNOLOGY,40(2),435-440. |
| MLA | Chen, Hongjian,et al."The involvement of cysteine-rich intestinal protein in early development and innate immunity of Asiatic hard clam, Meretrix meretrix".FISH & SHELLFISH IMMUNOLOGY 40.2(2014):435-440. |
入库方式： OAI收割
来源：海洋研究所
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