The protein expression profile in hepatopancreas of scallop Chlamys farreri under heat stress and Vibrio anguillarum challenge
文献类型：期刊论文
| 作者 | Sun, Zhibin1; Yang, Chuanyan1; Wang, Lingling1; Wang, Xingqiang1,3; Wang, Jingjing1,2 ; Yue, Feng1,2; Liu, Rui1; Zhang, Huan1; Song, Linsheng1
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| 刊名 | FISH & SHELLFISH IMMUNOLOGY
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| 出版日期 | 2014 |
| 卷号 | 36期号:1页码:252-260 |
| 关键词 | Chlamys farreri Hepatopancreas Heat stress Vibrio anguillarum challenge Proteome |
| 通讯作者 | Song, LS (reprint author), Chinese Acad Sci, Inst Oceanol, 7 Nanhai Rd, Qingdao 266071, Peoples R China. |
| 英文摘要 | Heat stress and pathogen infection have been considered as the main causes for mass mortality of cultured scallops during summer. In the present study, the expression profiles of proteins in the hepatopancreas of scallop Chlamys farreri were examined to reveal the possible mechanisms of physiological responses of scallop beneath heat stress and bacterial infection. An earlier occurred and higher mortality was observed in the scallops from combination treated group (28 degrees C and an injection of Vibrio anguillarum) in comparison to those in heat stress (28 degrees C) and bacteria challenge (V. anguillarum injection only) group, as well as control (PBS) and blank (untreated) group. The proteins in the hepatopancreas from scallops post 6 h of treatment were analyzed by using 2-D PAGE and ImageMaster 2D Platinum. There were total 1003 spots detected in control group, 1193 spots in heat stress group, 1263 spots in bacteria challenge group, and 1241 spots in the combination group. Fifteen protein spots expressed differentially between the combination treatment group and the bacteria challenge group were successfully identified by mass spectrometry and they were mainly classified as binding and catalytic proteins, such as endoglucanase, methylmalonate-semialdehyde dehydrogenase, xylose isomerase, tryptophanyl-tRNA synthetase, 40s ribosomal protein SA, glutathione S-transferase 4, and Mitochondrial transcription factor A, etc. These results indicated that the mortality of scallops suffered from the combination treatment was probably attributed to the impaired modulation of digestion and metabolism and ruined protein synthesis caused by heat stress together with bacteria infection. These data also provided valuable insights into the possible mechanisms of summer mortality occurrence of scallop at protein level. (C) 2013 Elsevier Ltd. All rights reserved. |
| 学科主题 | Fisheries; Immunology; Marine & Freshwater Biology; Veterinary Sciences |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000331671600030 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/24185]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China 3.Huaihai Inst Technol, Jiangsu Key Lab Marine Biotechnol, Lianyungang 222005, Peoples R China |
| 推荐引用方式 GB/T 7714 | Sun, Zhibin,Yang, Chuanyan,Wang, Lingling,et al. The protein expression profile in hepatopancreas of scallop Chlamys farreri under heat stress and Vibrio anguillarum challenge[J]. FISH & SHELLFISH IMMUNOLOGY,2014,36(1):252-260. |
| APA | Sun, Zhibin.,Yang, Chuanyan.,Wang, Lingling.,Wang, Xingqiang.,Wang, Jingjing.,...&Song, Linsheng.(2014).The protein expression profile in hepatopancreas of scallop Chlamys farreri under heat stress and Vibrio anguillarum challenge.FISH & SHELLFISH IMMUNOLOGY,36(1),252-260. |
| MLA | Sun, Zhibin,et al."The protein expression profile in hepatopancreas of scallop Chlamys farreri under heat stress and Vibrio anguillarum challenge".FISH & SHELLFISH IMMUNOLOGY 36.1(2014):252-260. |
入库方式： OAI收割
来源：海洋研究所
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