Transcriptome Analysis of the Initial Stage of Acute WSSV Infection Caused by Temperature Change
文献类型：期刊论文
| 作者 | Sun, Yumiao; Li, Fuhua; Sun, Zheng; Zhang, Xiaojun; Li, Shihao; Zhang, Chengsong ; Xiang, Jianhai
|
| 刊名 | PLOS ONE
 |
| 出版日期 | 2014-03-04 |
| 卷号 | 9期号:3页码:e90732 |
| 通讯作者 | Li, FH (reprint author), Chinese Acad Sci, Inst Oceanol, Qingdao, Peoples R China. |
| 英文摘要 | White spot syndrome virus (WSSV) is the most devastating virosis threatening the shrimp culture industry worldwide. Variations of environmental factors in shrimp culture ponds usually lead to the outbreak of white spot syndrome (WSS). In order to know the molecular mechanisms of WSS outbreak induced by temperature variation and the biological changes of the host at the initial stage of WSSV acute infection, RNA-Seq technology was used to analyze the differentially expressed genes (DEGs) in shrimp with a certain amount of WSSV cultured at 18 degrees C and shrimp whose culture temperature were raised to 25 degrees C. To analyze whether the expression changes of the DEGs were due to temperature rising or WSSV proliferation, the expression of selected DEGs was analyzed by real-time PCR with another shrimp group, namely Group T, as control. Group T didn't suffer WSSV infection but was subjected to temperature rising in parallel. At the initial stage of WSSV acute infection, DEGs related to energy production were up-regulated, whereas most DEGs related to cell cycle and positive regulation of cell death and were down-regulated. Triose phosphate isomerase, enolase and alcohol dehydrogenase involved in glycosis were up-regulated, while pyruvate dehydrogenase, citrate synthase and isocitrate dehydrogenase with NAD as the coenzyme involved in TCA pathway were down-regulated. Also genes involved in host DNA replication, including DNA primase, DNA topoisomerase and DNA polymerase showed down-regulated expression. Several interesting genes including crustin genes, acting binding or inhibiting protein genes, a disintegrin and metalloproteinase domain-containing protein 9 (ADAM9) gene and a GRP 78 gene were also analyzed. Understanding the interactions between hosts and WSSV at the initial stage of acute infection will not only help to get a deep insight into the pathogenesis of WSSV but also provide clues for therapies. |
| 学科主题 | Science & Technology - Other Topics |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000332475500093 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/24188]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 作者单位 | Chinese Acad Sci, Inst Oceanol, Qingdao, Peoples R China |
| 推荐引用方式 GB/T 7714 | Sun, Yumiao,Li, Fuhua,Sun, Zheng,et al. Transcriptome Analysis of the Initial Stage of Acute WSSV Infection Caused by Temperature Change[J]. PLOS ONE,2014,9(3):e90732. |
| APA | Sun, Yumiao.,Li, Fuhua.,Sun, Zheng.,Zhang, Xiaojun.,Li, Shihao.,...&Xiang, Jianhai.(2014).Transcriptome Analysis of the Initial Stage of Acute WSSV Infection Caused by Temperature Change.PLOS ONE,9(3),e90732. |
| MLA | Sun, Yumiao,et al."Transcriptome Analysis of the Initial Stage of Acute WSSV Infection Caused by Temperature Change".PLOS ONE 9.3(2014):e90732. |
入库方式： OAI收割
来源：海洋研究所
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