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Enhanced Production of Fatty Acids and Astaxanthin in Aurantiochytrium sp by the Expression of Vitreoscilla Hemoglobin

文献类型：期刊论文

作者Suen, Yung Lee1; Tang, Hongmei2,3; Huang, Junchao2; Chen, Feng4
刊名JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
出版日期2014-12-24
卷号62期号:51页码:12392-12398
关键词astaxanthin Aurantiochytrium fatty acid Vitreoscilla hemoglobin thraustochytrids
通讯作者Huang,JC (reprint author),Chinese Acad Sci,Kunming Inst Bot,Kunming,Peoples R China. ; huangjc@mail.kib.ac.cn ; sfchencoe@pku.edu.cn
英文摘要Dissolved oxygen is a critical factor for heterotrophic cell growth and metabolite production. The aim of this study was to investigate the effects of an oxygen-involved protein on cell growth and fatty acid and astaxanthin production in the biologically important thraustochytrid Aurantiochytrium sp. The hemoglobin of the Vitreoscilla stercoraria (VHb) gene was fused upstream with a zeocin resistance gene (ble) and driven by the Aurantiochytrium tubulin promoter. The expression construct was introduced into two strains of Aurantiochytrium sp. by electroporation. Transgenic Aurantiochytrium sp. strains MP4 and SK4 expressing the heterologous VHb achieved significantly higher maximum biomass than their corresponding controls in microaerobic conditions. Furthermore, the transformants of Aurantiochytrium sp. SK4 produced 44% higher total fatty acid and 9-fold higher astaxanthin contents than the wild type control in aerobic conditions. The present study highlights the biotechnological application of VHb in high-cell density fermentation for enhanced biomass production as well as high-value metabolites.
学科主题Agriculture, Multidisciplinary; Chemistry, Applied; Food Science & Technology
类目[WOS]Agriculture, Multidisciplinary ; Chemistry, Applied ; Food Science & Technology
研究领域[WOS]Agriculture ; Chemistry ; Food Science & Technology
关键词[WOS]GENE PHYLOGENY THRAUSTOCHYTRIACEAE ; TAXONOMIC REARRANGEMENT ; SCHIZOCHYTRIUM ; OPTIMIZATION ; XANTHOPHYLLS ; EMENDATION ; MORPHOLOGY ; ERECTION ; SQUALENE ; NOV
收录类别SCI
语种英语
WOS记录号WOS:000347138500015
源URL[http://ir.kib.ac.cn/handle/151853/19376]  
专题昆明植物研究所_资源植物与生物技术所级重点实验室
作者单位1.Univ Hong Kong,Pokfulam, Sch Biol Sci, Hong Kong, Hong Kong, Peoples R China
2.Chinese Acad Sci, Kunming Inst Bot, Kunming, Peoples R China
3.Univ Chinese Acad Sci, Beijing, Peoples R China
4.Peking Univ, Coll Engn, Inst Food & Bioresource Engn, Beijing 100871, Peoples R China
推荐引用方式
GB/T 7714		 Suen, Yung Lee,Tang, Hongmei,Huang, Junchao,et al. Enhanced Production of Fatty Acids and Astaxanthin in Aurantiochytrium sp by the Expression of Vitreoscilla Hemoglobin[J]. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY,2014,62(51):12392-12398.
APA Suen, Yung Lee,Tang, Hongmei,Huang, Junchao,&Chen, Feng.(2014).Enhanced Production of Fatty Acids and Astaxanthin in Aurantiochytrium sp by the Expression of Vitreoscilla Hemoglobin.JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY,62(51),12392-12398.
MLA Suen, Yung Lee,et al."Enhanced Production of Fatty Acids and Astaxanthin in Aurantiochytrium sp by the Expression of Vitreoscilla Hemoglobin".JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY 62.51(2014):12392-12398.

入库方式： OAI收割

来源：昆明植物研究所

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