Sub-nanometer drift correction for super-resolution imaging
文献类型：期刊论文
| 作者 | Tang, Y ; Wang, X ; Zhang, X ; Li, J ; Dai, L |
| 刊名 | OPTICS LETTERS
 |
| 出版日期 | 2014 |
| 卷号 | 39期号:19页码:5685-5688 |
| 关键词 | PHOTOACTIVATION LOCALIZATION MICROSCOPY OPTICAL MICROSCOPE FLUORESCENCE RECONSTRUCTION REGISTRATION NM |
| ISSN号 | 0146-9592 |
| 通讯作者 | Tang, Y (reprint author), Chongqing Univ, Dept Phys, Chongqing 400044, Peoples R China. |
| 英文摘要 | Spatial resolution of conventional far-field fluorescence microscopy is limited by diffraction of light. Single-molecule localization microscopy (SMLM), such as (direct) stochastic optical reconstruction microscopy (dSTORM/STORM), and (fluorescence) photoactivation localization microscopy (fPALM/PALM), can break this barrier by localizing single emitters and reconstructing super-resolution image with much higher precision. Nevertheless, a SMLM measurement needs to record a large number of image frames and takes considerable recording time. In this process, sample drift becomes a critical problem and cannot be neglected. In this Letter, we present a sub-nanometer precision, low-cost sample drift correction method based on minimizing normalized root-mean-square error (NRMSE) between bright field images. Two optical configurations are suggested for recording bright field and fluorescence images simultaneously or alternately. The method was demonstrated on simulated data, and better than 0.3 nm drift correction precision was achieved. It was also applied on dSTORM imaging of F-actins of 3T3 cell, and the quality of reconstructed super-resolution image was improved observably. This method does not require special hardware, extra labelling or markers, and no precision decline due to photobleaching. It can be applied as an add-on for SMLM setups and achieves sub-nanometer precision drift correction for post-measurement or real time drift compensation. (C) 2014 Optical Society of America |
| 学科主题 | Physics |
| 收录类别 | SCI |
| 资助信息 | National Natural Science Foundation of China [21273053]; National Basic Research Program of China (973 program) [2013CB932804]; Major Research Plan of the National Natural Science Foundation of China [91027045] |
| 原文出处 | http://dx.doi.org/10.1364/OL.39.005685 |
| 语种 | 英语 |
| WOS记录号 | WOS:000343906400055 |
| 公开日期 | 2015-06-03 |
| 源URL | [http://ir.itp.ac.cn/handle/311006/15643]  |
| 专题 | 理论物理研究所_理论物理所1978-2010年知识产出 |
| 推荐引用方式 GB/T 7714 | Tang, Y,Wang, X,Zhang, X,et al. Sub-nanometer drift correction for super-resolution imaging[J]. OPTICS LETTERS,2014,39(19):5685-5688. |
| APA | Tang, Y,Wang, X,Zhang, X,Li, J,&Dai, L.(2014).Sub-nanometer drift correction for super-resolution imaging.OPTICS LETTERS,39(19),5685-5688. |
| MLA | Tang, Y,et al."Sub-nanometer drift correction for super-resolution imaging".OPTICS LETTERS 39.19(2014):5685-5688. |
入库方式： OAI收割
来源：理论物理研究所
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