Mechano-chemical selections of two competitive unfolding pathways of a single DNA i-motif
文献类型：期刊论文
| 作者 | Xu, Y; Chen, H; Qu, YJ; Efremov, AK; Li, M ; Ouyang, ZC; Liu, DS; Yan, J
|
| 刊名 | CHINESE PHYSICS B
 |
| 出版日期 | 2014 |
| 卷号 | 23期号:6页码:68702 |
| 关键词 | G-QUADRUPLEX MECHANICAL STABILITY RNA-POLYMERASE STRANDED-DNA DOUBLE HELIX B-DNA MOLECULE FORCE PROTEINS DYNAMICS |
| ISSN号 | 1674-1056 |
| 通讯作者 | Liu, DS (reprint author), Tsinghua Univ, Minist Educ, Dept Chem, Key Lab Organ Optoelect & Mol Engn, Beijing 100084, Peoples R China. |
| 英文摘要 | The DNA i-motif is a quadruplex structure formed in tandem cytosine-rich sequences in slightly acidic conditions. Besides being considered as a building block of DNA nano-devices, it may also play potential roles in regulating chromosome stability and gene transcriptions. The stability of i-motif is crucial for these functions. In this work, we investigated the mechanical stability of a single i-motif formed in the human telomeric sequence 5'-(CCCTAA)(3)CCC, which revealed a novel pH and loading rate-dependent bimodal unfolding force distribution. Although the cause of the bimodal unfolding force species is not clear, we proposed a phenomenological model involving a direct unfolding favored at lower loading rate or higher pH value, which is subject to competition with another unfolding pathway through a mechanically stable intermediate state whose nature is yet to be determined. Overall, the unique mechano-chemical responses of i-motif-provide a new perspective to its stability, which may be useful to guide designing new i-motif-based DNA mechanical nano-devices. |
| 学科主题 | Physics |
| 收录类别 | SCI |
| 资助信息 | National Research Foundation through the Mechanobiology Institute Singapore and the Ministry of Education of Singapore [MOE2012-T3-1-001]; National Basic Research Program of China [2013CB932800]; Major Research Plan of the National Natural Science Foundation of China [91027046, 91027045]; Fundamental Research Funds for the Central Universities [2013121005] |
| 原文出处 | http://dx.doi.org/10.1088/1674-1056/23/6/068702 |
| 语种 | 英语 |
| WOS记录号 | WOS:000338668200102 |
| 公开日期 | 2015-06-03 |
| 源URL | [http://ir.itp.ac.cn/handle/311006/15749]  |
| 专题 | 理论物理研究所_理论物理所1978-2010年知识产出 |
| 推荐引用方式 GB/T 7714 | Xu, Y,Chen, H,Qu, YJ,et al. Mechano-chemical selections of two competitive unfolding pathways of a single DNA i-motif[J]. CHINESE PHYSICS B,2014,23(6):68702. |
| APA | Xu, Y.,Chen, H.,Qu, YJ.,Efremov, AK.,Li, M.,...&Yan, J.(2014).Mechano-chemical selections of two competitive unfolding pathways of a single DNA i-motif.CHINESE PHYSICS B,23(6),68702. |
| MLA | Xu, Y,et al."Mechano-chemical selections of two competitive unfolding pathways of a single DNA i-motif".CHINESE PHYSICS B 23.6(2014):68702. |
入库方式： OAI收割
来源：理论物理研究所
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