PDMP induces rapid changes in vacuole morphology in Arabidopsis root cells
文献类型：期刊论文
| 作者 | Kruger, F. |
| 刊名 | Journal of Experimental Botany
 |
| 出版日期 | 2013 |
| 卷号 | 64期号:2页码:529-540 |
| ISSN号 | 0022-0957 |
| 中文摘要 | PDMP (d-l-threo-1-phenyl-2-decanoyl amino-3-morpholino-1-propanol) is a well-known inhibitor of glucosylceramide synthase (GCS), a key enzyme in sphingolipid biosynthesis. Through the resultant increase in ceramides which interact with mTOR and Beclin1 (Atg6), this drug is also known to induce macroautophagy in mammalian cells. This study investigated the response of Arabidopsis root cells to PDMP, and what are probably numerous tightly packed small vacuoles in the control cells appear to fuse to form a single globular-shaped vacuole. However, during this fusion process, cytoplasm channels between the individual vacuoles become trapped in deep invaginations of the tonoplast. In both optical sections in the confocal laser scanning microscope and in ultrathin sections in the electron microscope, these invaginations have the appearance of cytoplasmic inclusions in the vacuole lumen. These changes in vacuole morphology are rapid (occurring within minutes after application of PDMP) and are independent of ongoing protein synthesis. The tonoplast invaginations remain visible for hours, but after 24h almost all disappear. Experiments designed to examine whether ceramide levels might be the cause of the PDMP effect have not proved conclusive. On the other hand, this study has been able to rule out the release of Ca-2 ions from intracellular stores as a contributing factor. |
| 语种 | 英语 |
| 公开日期 | 2015-06-08 |
| 源URL | [http://ir.xtbg.org.cn/handle/353005/8783]  |
| 专题 | 西双版纳热带植物园_公共技术服务中心主要仪器相关文献_激光共聚焦显微镜 |
| 推荐引用方式 GB/T 7714 | Kruger, F.. PDMP induces rapid changes in vacuole morphology in Arabidopsis root cells[J]. Journal of Experimental Botany,2013,64(2):529-540. |
| APA | Kruger, F..(2013).PDMP induces rapid changes in vacuole morphology in Arabidopsis root cells.Journal of Experimental Botany,64(2),529-540. |
| MLA | Kruger, F.."PDMP induces rapid changes in vacuole morphology in Arabidopsis root cells".Journal of Experimental Botany 64.2(2013):529-540. |
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来源：西双版纳热带植物园
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