Vector alkaline phosphatase substrate Blue III: one substrate for brightfield histochemistry and high-resolution fluorescence imaging by confocal laser scanning microscopy
文献类型：期刊论文
| 作者 | Hollinshead, M. |
| 刊名 | Histochemical Journal
 |
| 出版日期 | 1998 |
| 卷号 | 30期号:8页码:577-581 |
| ISSN号 | 0018-2214 |
| 中文摘要 | A number of histochemical chromogenic substrates for alkaline phosphatase are commercially available and give reaction products with a range of colours for brightfield examination. Some of these reaction products are also fluorescent, exhibiting a wide excitation range and a broad emission peak. We report here that one of these substrates, Vector Blue III, yields a stable, strongly fluorescent reaction product with an excitation peak around 500 nm and a large Stokes shift to an emission peak at 680 nm. The reaction product can be excited using a mercury lamp with a fluorescein excitation filter or an argon ion laser at 488 nm or 568 nm, and the emission detected using a long-pass filter designed for Cy-5. Thus, a single substrate is suitable for brightfield imaging of tissue sections and high-resolution analysis of subcellular detail, using a confocal laser scanning microscope, in the same specimen. (C) 1998 Chapman & Hall. |
| 语种 | 英语 |
| 公开日期 | 2015-06-08 |
| 源URL | [http://ir.xtbg.org.cn/handle/353005/8794]  |
| 专题 | 西双版纳热带植物园_公共技术服务中心主要仪器相关文献_激光共聚焦显微镜 |
| 推荐引用方式 GB/T 7714 | Hollinshead, M.. Vector alkaline phosphatase substrate Blue III: one substrate for brightfield histochemistry and high-resolution fluorescence imaging by confocal laser scanning microscopy[J]. Histochemical Journal,1998,30(8):577-581. |
| APA | Hollinshead, M..(1998).Vector alkaline phosphatase substrate Blue III: one substrate for brightfield histochemistry and high-resolution fluorescence imaging by confocal laser scanning microscopy.Histochemical Journal,30(8),577-581. |
| MLA | Hollinshead, M.."Vector alkaline phosphatase substrate Blue III: one substrate for brightfield histochemistry and high-resolution fluorescence imaging by confocal laser scanning microscopy".Histochemical Journal 30.8(1998):577-581. |
入库方式： OAI收割
来源：西双版纳热带植物园
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