Three-dimensional visualization analysis of in vitro cultured bone fabricated by rat marrow mesenchymal stem cells
文献类型：期刊论文
| 作者 | Kihara, T. |
| 刊名 | Biochemical and Biophysical Research Communications
 |
| 出版日期 | 2004 |
| 卷号 | 316期号:3页码:943-948 |
| ISSN号 | 0006-291X |
| 中文摘要 | Marrow mesenchymal stem cells are well known for their differentiation into bone-forming osteoblasts and in vitro mineralized tissue formation. However, process details, including tissue structure and cellular environments, remain unclear. The present study demonstrates three-dimensional visualization of tissue fabricated by culturing MSCs in the presence of calcein, a fluorescent marker for bone mineralization. The 3D visualization was performed by computer-assisted confocal laser scanning microscopy and revealed that the in vitro tissue consisted of layers of a mineralized matrix with round cells in the matrix lacunae, an unmineralized matrix (osteoid), and osteoblastic cells on the osteoid surface. The findings show that the mineralization by cultured MSCs is an in vitro counterpart of in vivo bone formation and indicate that the novel technique of visualization without tissue fixation could be useful for continuous monitoring of tissue organization in an ongoing culture. (C) 2004 Elsevier Inc. All rights reserved. |
| 语种 | 英语 |
| 公开日期 | 2015-06-08 |
| 源URL | [http://ir.xtbg.org.cn/handle/353005/8878]  |
| 专题 | 西双版纳热带植物园_公共技术服务中心主要仪器相关文献_激光共聚焦显微镜 |
| 推荐引用方式 GB/T 7714 | Kihara, T.. Three-dimensional visualization analysis of in vitro cultured bone fabricated by rat marrow mesenchymal stem cells[J]. Biochemical and Biophysical Research Communications,2004,316(3):943-948. |
| APA | Kihara, T..(2004).Three-dimensional visualization analysis of in vitro cultured bone fabricated by rat marrow mesenchymal stem cells.Biochemical and Biophysical Research Communications,316(3),943-948. |
| MLA | Kihara, T.."Three-dimensional visualization analysis of in vitro cultured bone fabricated by rat marrow mesenchymal stem cells".Biochemical and Biophysical Research Communications 316.3(2004):943-948. |
入库方式： OAI收割
来源：西双版纳热带植物园
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