SPECTRA AND FLUORESCENCE LIFETIMES OF LISSAMINE RHODAMINE, TETRAMETHYLRHODAMINE ISOTHIOCYANATE, TEXAS RED, AND CYANINE-3.18 FLUOROPHORES - INFLUENCES OF SOME ENVIRONMENTAL-FACTORS RECORDED WITH A CONFOCAL LASER-SCANNING MICROSCOPE
文献类型：期刊论文
| 作者 | Brismar, H. |
| 刊名 | Journal of Histochemistry & Cytochemistry
 |
| 出版日期 | 1995 |
| 卷号 | 43期号:7页码:699-707 |
| ISSN号 | 0022-1554 |
| 中文摘要 | We report on the spectra and fluorescence lifetimes of four commonly used fluorophores: lissamine rhodamine (LRSC); tetramethyl rhodamine isothiocyanate (TRITC); Texas Red; and cyanine 3.18 (Cy-3), Fluorescence lifetime recordings revealed that these spectrally overlapping fluorophores can be individually detected by their lifetimes, indicating that at least four fluorophores can be individually identified in discrete tissue domains by confocal microscopy. A further advantage of lifetime recordings is that fluorophores that emit light within the same wavelength band can be used and chromatic aberrations are therefore circumvented, thereby improving the spatial accuracy in imaging of multiple fluorophores, Low and high pH, respectively, tended to influence fluorophore emission spectra and fluorescence lifetime, IgG conjugation of the fluorophores tended to shift the spectra towards longer wavelengths and to change the fluorescence lifetimes. The IgG-conjugated form of the fluorophores may, when applied to tissue specimens, change the emission spectrum and lifetime. In addition, different tissue embedding procedures may influence fluorescence lifetime, These observations emphasize the importance of spectral and lifetime characterization of fluorescent probes within the chemical context in which they will be used experimentally. Changes in spectra and fluorescence lifetimes may be a useful tool to gain information about the chemical environment of the fluorophores. |
| 语种 | 英语 |
| 公开日期 | 2015-06-08 |
| 源URL | [http://ir.xtbg.org.cn/handle/353005/8951]  |
| 专题 | 西双版纳热带植物园_公共技术服务中心主要仪器相关文献_激光共聚焦显微镜 |
| 推荐引用方式 GB/T 7714 | Brismar, H.. SPECTRA AND FLUORESCENCE LIFETIMES OF LISSAMINE RHODAMINE, TETRAMETHYLRHODAMINE ISOTHIOCYANATE, TEXAS RED, AND CYANINE-3.18 FLUOROPHORES - INFLUENCES OF SOME ENVIRONMENTAL-FACTORS RECORDED WITH A CONFOCAL LASER-SCANNING MICROSCOPE[J]. Journal of Histochemistry & Cytochemistry,1995,43(7):699-707. |
| APA | Brismar, H..(1995).SPECTRA AND FLUORESCENCE LIFETIMES OF LISSAMINE RHODAMINE, TETRAMETHYLRHODAMINE ISOTHIOCYANATE, TEXAS RED, AND CYANINE-3.18 FLUOROPHORES - INFLUENCES OF SOME ENVIRONMENTAL-FACTORS RECORDED WITH A CONFOCAL LASER-SCANNING MICROSCOPE.Journal of Histochemistry & Cytochemistry,43(7),699-707. |
| MLA | Brismar, H.."SPECTRA AND FLUORESCENCE LIFETIMES OF LISSAMINE RHODAMINE, TETRAMETHYLRHODAMINE ISOTHIOCYANATE, TEXAS RED, AND CYANINE-3.18 FLUOROPHORES - INFLUENCES OF SOME ENVIRONMENTAL-FACTORS RECORDED WITH A CONFOCAL LASER-SCANNING MICROSCOPE".Journal of Histochemistry & Cytochemistry 43.7(1995):699-707. |
入库方式： OAI收割
来源：西双版纳热带植物园
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。