PURIFICATION OF A LOW-MOLECULAR-WEIGHT MICROTUBULE-BINDING PROTEIN FROM SEA-URCHIN EGGS
文献类型：期刊论文
| 作者 | Maekawa, S. |
| 刊名 | Biochimica Et Biophysica Acta-Protein Structure and Molecular Enzymology
 |
| 出版日期 | 1994 |
| 卷号 | 1207期号:2页码:194-200 |
| ISSN号 | 0167-4838 |
| 中文摘要 | A low molecular weight microtubule binding protein(SU-MAP34) was purified from sea urchin eggs. This protein bound strongly to the microtubule formed from purified echinoderm tubulin but showed no cross-linking of microtubules. Monospecific antibody against SU-MAP34 was produced and an immunoblotting analysis showed that this protein was not a breakdown product of a protein of a higher molecular mass. Whole cell staining and confocal laser scanning microscope observation showed that SU-MAP34 localized on the filamentous structure of mitotic apparatus and this structure was identified as the microtubule with double staining using anti-SU-MAP34 and anti-tubulin. An immunoblotting experiment showed an enrichment of SU-MAP34 in a microtubule protein fraction prepared using taxol from a crude extract of the cell. |
| 语种 | 英语 |
| 公开日期 | 2015-06-08 |
| 源URL | [http://ir.xtbg.org.cn/handle/353005/8955]  |
| 专题 | 西双版纳热带植物园_公共技术服务中心主要仪器相关文献_激光共聚焦显微镜 |
| 推荐引用方式 GB/T 7714 | Maekawa, S.. PURIFICATION OF A LOW-MOLECULAR-WEIGHT MICROTUBULE-BINDING PROTEIN FROM SEA-URCHIN EGGS[J]. Biochimica Et Biophysica Acta-Protein Structure and Molecular Enzymology,1994,1207(2):194-200. |
| APA | Maekawa, S..(1994).PURIFICATION OF A LOW-MOLECULAR-WEIGHT MICROTUBULE-BINDING PROTEIN FROM SEA-URCHIN EGGS.Biochimica Et Biophysica Acta-Protein Structure and Molecular Enzymology,1207(2),194-200. |
| MLA | Maekawa, S.."PURIFICATION OF A LOW-MOLECULAR-WEIGHT MICROTUBULE-BINDING PROTEIN FROM SEA-URCHIN EGGS".Biochimica Et Biophysica Acta-Protein Structure and Molecular Enzymology 1207.2(1994):194-200. |
入库方式： OAI收割
来源：西双版纳热带植物园
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