A novel magneto-DNA duplex probe for bacterial DNA detection based on exonuclease III-aided cycling amplification
文献类型：期刊论文
| 作者 | Zeng, Yan1,2 ; Wan, Yi1; Zhang, Dun1; Qi, Peng1,2
|
| 刊名 | TALANTA
 |
| 出版日期 | 2015-01-15 |
| 卷号 | 132页码:59-64 |
| 关键词 | Magneto-DNA duplex probe Magnetic microparticle Exonuclease III Bacterial DNA detection |
| 英文摘要 | A novel magneto-DNA duplex probe for bacterial DNA detection based on exonuclease HI (Exo-III) aided cycling amplification has been developed. This magneto-DNA duplex probe contains a partly hybrid fluorophore-modified capture probe and a fluorophore-modified signal probe with magnetic microparticle as carrier. In the presence of a perfectly matched target bacterial DNA, blunt 3'-terminus of the capture probe is formed, activating the Exo-III aided cycling amplification. Thus, Exo-III catalyzes the stepwise removal of mononucleotides from this terminus, releasing both fluorophore-modified signal probe, fluorescent dyes of the capture probe and target DNA. The released target DNA then starts a new cycle, while released fluorescent fragments are recovered with magnetic separation for fluorescence signal collection. This system exhibited sensitive detection of bacterial DNA, with a detection limit of 14 pM because of the unique cleavage function of Exo-III, high fluorescence intensity, and separating function of magneto-DNA duplex probes. Besides this sensitivity, this strategy exhibited excellent selectivity with mismatched bacterial DNA targets and other bacterial species targets and good applicability in real seawater samples, hence, this strategy could be potentially used for qualitative and quantitative analysis of bacteria. (C) 2014 Elsevier B.V. All rights reserved. |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000349278900009 |
| 公开日期 | 2015-06-16 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/25364]  |
| 专题 | 海洋研究所_海洋腐蚀与防护研究发展中心 |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Key Lab Marine Environm Corros & Biofouling, Qingdao 266071, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100039, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zeng, Yan,Wan, Yi,Zhang, Dun,et al. A novel magneto-DNA duplex probe for bacterial DNA detection based on exonuclease III-aided cycling amplification[J]. TALANTA,2015,132:59-64. |
| APA | Zeng, Yan,Wan, Yi,Zhang, Dun,&Qi, Peng.(2015).A novel magneto-DNA duplex probe for bacterial DNA detection based on exonuclease III-aided cycling amplification.TALANTA,132,59-64. |
| MLA | Zeng, Yan,et al."A novel magneto-DNA duplex probe for bacterial DNA detection based on exonuclease III-aided cycling amplification".TALANTA 132(2015):59-64. |
入库方式： OAI收割
来源：海洋研究所
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