中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
Cultivation of the benthic microalga Prorocentrum lima for the production of diarrhetic shellfish poisoning toxins in a vertical flat photobioreactor

文献类型:期刊论文

作者Wang, Shuai1,2; Chen, Junhui1; Li, Zhaoyong1; Wang, Yanlong1; Fu, Boqiang3; Han, Xiaotian4; Zheng, Li1
刊名BIORESOURCE TECHNOLOGY
出版日期2015-03-01
卷号179页码:243-248
关键词Benthic microalgal cultivation Okadaic acid Dinophysistoxin-1 Photobioreactor Microalgal harvesting
英文摘要In this study, the cultivation conditions of Prorocentrum lima, including temperature, nutrient concentration, photoperiod, and salinity were observed, and then an effective method for the large-volume cultivation of P. lima using a vertical flat photobioreactor was developed for the first time. The maximum cell concentrations and toxin contents of P. lima cultured in the photobioreactor were reached after a 35 days cultivation. Moreover, a step-wise double-sedimentation centrifugation method was used to harvest the microalgae cells, with the harvest rate of 89%. Toxin analysis of dry microalgal powder indicated that OA and DTX1 contents were 15.2 and 21.6 mg g(-1), respectively. These results verify that the culture method using the proposed photobioreactor is effective to massively produce DSP toxin-containing P. lima. This study may serve as a guide for the large-scale production of toxin-producing red-tide benthic microalgae. (C) 2014 Elsevier Ltd. All rights reserved.
收录类别SCI
语种英语
WOS记录号WOS:000348041600034
公开日期2015-06-16
源URL[http://ir.qdio.ac.cn/handle/337002/25346]  
专题海洋研究所_海洋生态与环境科学重点实验室
作者单位1.State Ocean Adm, Inst Oceanog 1, Res Ctr Marine Ecol, Qingdao 266061, Peoples R China
2.Ocean Univ China, Coll Marine Life Sci, Qingdao 266003, Peoples R China
3.Natl Inst Metrol, Div Med & Biol Measurements, Lab Biol Anal & Cell Technol, Beijing 100013, Peoples R China
4.Chinese Acad Sci, Inst Oceanol, Key Lab Marine Ecol & Environm Sci, Qingdao 266071, Peoples R China
推荐引用方式
GB/T 7714
Wang, Shuai,Chen, Junhui,Li, Zhaoyong,et al. Cultivation of the benthic microalga Prorocentrum lima for the production of diarrhetic shellfish poisoning toxins in a vertical flat photobioreactor[J]. BIORESOURCE TECHNOLOGY,2015,179:243-248.
APA Wang, Shuai.,Chen, Junhui.,Li, Zhaoyong.,Wang, Yanlong.,Fu, Boqiang.,...&Zheng, Li.(2015).Cultivation of the benthic microalga Prorocentrum lima for the production of diarrhetic shellfish poisoning toxins in a vertical flat photobioreactor.BIORESOURCE TECHNOLOGY,179,243-248.
MLA Wang, Shuai,et al."Cultivation of the benthic microalga Prorocentrum lima for the production of diarrhetic shellfish poisoning toxins in a vertical flat photobioreactor".BIORESOURCE TECHNOLOGY 179(2015):243-248.

入库方式: OAI收割

来源:海洋研究所

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