Hydrogen production of a salt tolerant strain Bacillus sp. B2 from marine intertidal sludge
文献类型：期刊论文
| 作者 | Liu, Hongyan1,2; Wang, Guangce1 |
| 刊名 | WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY
 |
| 出版日期 | 2012 |
| 卷号 | 28期号:1页码:31-37 |
| 关键词 | Intertidal zone sludge Denaturing gradient gel electrophoresis (DGGE) Bacillus sp. Hydrogen production |
| 英文摘要 | To isolate a salt tolerant hydrogen-producing bacterium, we used the sludge from the intertidal zone of a bathing beach in Tianjin as inoculum to enrich hydrogen-producing bacteria. The sludge was treated by heat-shock pretreatment with three different temperature (80, 100 and 121A degrees C) respectively. A hydrogen-producing bacterium was isolated from the sludge pretreated at 80A degrees C by sandwich plate technique and identified using microscopic examination and 16S rDNA gene sequence analysis. The isolated bacterium was named as Bacillus sp. B2. The present study examined the hydrogen-producing ability of Bacillus sp. B2. The strain was able to produce hydrogen over a wide range of initial pH from 5.0 to 10.0, with an optimum at pH 7.0. The level of hydrogen production was also affected by the salt concentration. Strain B2 has unique capability to adapt high salt concentration. It could produce hydrogen at the salt concentration from 4 to 60aEuro degrees. The maximum of hydrogen-producing yield of strain B2 was 1.65 +/- A 0.04 mol H-2/mol glucose (mean +/- A SE) at an initial pH value of 7.0 in marine culture conditions. Hydrogen production under fresh culture conditions reached a higher level than that in marine ones. As a result, it is likely that Bacillus sp. B2 could be applied to biohydrogen production using both marine and fresh organic waste. |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000298599700005 |
| 公开日期 | 2015-06-16 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/25336]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 2.Univ Sci & Technol, Coll Marine Sci & Engn, Tianjin Key Lab Marine Resources & Chem, Tianjin 300457, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu, Hongyan,Wang, Guangce. Hydrogen production of a salt tolerant strain Bacillus sp. B2 from marine intertidal sludge[J]. WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY,2012,28(1):31-37. |
| APA | Liu, Hongyan,&Wang, Guangce.(2012).Hydrogen production of a salt tolerant strain Bacillus sp. B2 from marine intertidal sludge.WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY,28(1),31-37. |
| MLA | Liu, Hongyan,et al."Hydrogen production of a salt tolerant strain Bacillus sp. B2 from marine intertidal sludge".WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY 28.1(2012):31-37. |
入库方式： OAI收割
来源：海洋研究所
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