Immune effects of R848: Evidences that suggest an essential role of TLR7/8-induced, Myd88-and NF-kappa B-dependent signaling in the antiviral immunity of Japanese flounder (Paralichthys olivaceus)
文献类型：期刊论文
| 作者 | Zhou, Zhi-xia; Sun, Li |
| 刊名 | DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
 |
| 出版日期 | 2015-03-01 |
| 卷号 | 49期号:1页码:113-120 |
| 关键词 | R848 Toll-like receptor Myd88 NK-kappa B Teleost Antiviral immunity |
| 英文摘要 | The imidazoquinoline compound R848 is a specific agonist of toll-like receptor (TLR) 7/TLR8 that has been used as an immunostimulant in humans against viral diseases. Although R848-induced immune response has been reported in teleost fish, the relevant mechanism is not clear. In this study, we investigated the antiviral potential and the signaling pathway of R848 in a model of Japanese flounder (Paralichthys olivaceus). We found that R848 was able to inhibit the replication of megalocytivirus, stimulated the proliferation of peripheral blood leukocytes (PBL), enhanced the expression of immune genes, and reduced apoptosis of PBL. When endosomal acidification was blocked by chloroquine (CQ), R848-mediated antiviral activity and immune response were significantly reduced. Likewise, inhibition of Myd88 activation markedly impaired the pro-proliferation and anti-apoptosis effect of R848. Cellular study showed that cultured founder cells treated with R848 exhibited augmented NF-kappa B activity, which, however, was dramatically reduced in the presence of CQand Myd88 inhibitor. Furthermore, when NF-kappa B was inactivated, the effect of R848 on cell proliferation and apoptosis was significantly decreased. Taken together, these results indicate that R848 is an immunostimulant with antiviral property in a teleost species, and that the immune response of R848 is mediated by, most likely, TLR7/TLR8 signaling pathway, in which Myd88 and NK--kappa B play an essential role. (C) 2014 Elsevier Ltd. All rights reserved. |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000348889800013 |
| 公开日期 | 2015-06-16 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/25368]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 作者单位 | Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhou, Zhi-xia,Sun, Li. Immune effects of R848: Evidences that suggest an essential role of TLR7/8-induced, Myd88-and NF-kappa B-dependent signaling in the antiviral immunity of Japanese flounder (Paralichthys olivaceus)[J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2015,49(1):113-120. |
| APA | Zhou, Zhi-xia,&Sun, Li.(2015).Immune effects of R848: Evidences that suggest an essential role of TLR7/8-induced, Myd88-and NF-kappa B-dependent signaling in the antiviral immunity of Japanese flounder (Paralichthys olivaceus).DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,49(1),113-120. |
| MLA | Zhou, Zhi-xia,et al."Immune effects of R848: Evidences that suggest an essential role of TLR7/8-induced, Myd88-and NF-kappa B-dependent signaling in the antiviral immunity of Japanese flounder (Paralichthys olivaceus)".DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY 49.1(2015):113-120. |
入库方式： OAI收割
来源：海洋研究所
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