A novel phagocytic receptor (CgNimC) from Pacific oyster Crassostrea gigas with lipopolysaccharide and gram-negative bacteria binding activity
文献类型：期刊论文
| 作者 | Wang, Weilin1,2 ; Liu, Rui1; Zhang, Tao1,2; Zhang, Ran3; Song, Xuan4; Wang, Lingling1; Song, Linsheng1
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| 刊名 | FISH & SHELLFISH IMMUNOLOGY
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| 出版日期 | 2015-03-01 |
| 卷号 | 43期号:1页码:103-110 |
| 关键词 | Phagocytic receptor Nimrod Lipopolysaccharide Crassostrea gigas Immune response |
| 英文摘要 | Phagocytosis is an evolutionarily conserved process to ingest the invading microbes and apoptotic or necrotic corpses, playing vital roles in defensing invaders and maintenance of normal physiological conditions. In the present study, a new Nimrod family phagocytic receptor with three EGF-like domains was identified in Pacific oyster Crassostrea gigas (designated CgNimC). CgNimC shared homology with other identified multiple EGF-like domain containing proteins. The mRNA transcripts of CgNimC were mainly distributed in mantle and hemocytes. Its relative expression level in hemocytes was significantly (P < 0.01) up-regulated after the injection of bacteria Vibrio anguillarum. Different to the NimC in Drosophila and Anopheles gambiae, the recombinant protein of CgNimC (rCgNimC) could bind directly to two gram-negative bacteria V. anguillarum and Vibrio splendidus, but not to gram-positive bacteria Staphylococci aureus, Micrococcus luteus or fungi Yarrowia lipolytica and Pichia pastoris. The affinity of rCgNimC toward M. luteus and Y. lipolytica was enhanced when the microorganisms were pre-incubated with the cell free hemolymph. rCgNimC exhibited higher affinity to lipopolysaccharide (LPS) and relatively lower affinity to peptidoglycan (PGN), while no affinity to glucan (GLU). After the CgNimC receptor was blocked by anti-rCgNimC antibody in vitro, the phagocytic rate of hemocytes toward two gram-negative bacteria V. anguillarum and V. splendidus was reduced significantly (P < 0.05), but no significant change of phagocytic rate was observed toward M. luteus and Y. lipolytica. All these results implied that CgNimC, with significant binding capability to LPS and gram-negative bacteria, was a novel phagocytic receptor involved in immune response of Pacific oyster. Further, it was speculated that receptors of Nimrod family might function as a phagocytic receptor to recognize PAMPs on the invaders and its recognition could be promoted by opsonization of molecules in hemolymph. (C) 2014 Elsevier Ltd. All rights reserved. |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000349731400012 |
| 公开日期 | 2015-06-16 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/25374]  |
| 专题 | 海洋研究所_海洋生态与环境科学重点实验室 海洋研究所_实验海洋生物学重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Ningbo Univ, Ningbo 315211, Zhejiang, Peoples R China 4.Sun Yat Sen Univ, Guangzhou 510275, Guangdong, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Weilin,Liu, Rui,Zhang, Tao,et al. A novel phagocytic receptor (CgNimC) from Pacific oyster Crassostrea gigas with lipopolysaccharide and gram-negative bacteria binding activity[J]. FISH & SHELLFISH IMMUNOLOGY,2015,43(1):103-110. |
| APA | Wang, Weilin.,Liu, Rui.,Zhang, Tao.,Zhang, Ran.,Song, Xuan.,...&Song, Linsheng.(2015).A novel phagocytic receptor (CgNimC) from Pacific oyster Crassostrea gigas with lipopolysaccharide and gram-negative bacteria binding activity.FISH & SHELLFISH IMMUNOLOGY,43(1),103-110. |
| MLA | Wang, Weilin,et al."A novel phagocytic receptor (CgNimC) from Pacific oyster Crassostrea gigas with lipopolysaccharide and gram-negative bacteria binding activity".FISH & SHELLFISH IMMUNOLOGY 43.1(2015):103-110. |
入库方式： OAI收割
来源：海洋研究所
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