中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
Molecular markers for identifying a new selected variety of Pacific white shrimp Litopenaeus vannamei

文献类型:期刊论文

作者Yu Yang1,2; Zhang Xiaojun1; Liu Jingwen1,2; Li Fuhua1; Huang Hao3; Li Yijun3; Liu Xiaolin4; Xiang Jianhai1
刊名CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY
出版日期2015
卷号33期号:1页码:1-10
关键词molecular identification Litopenaeus vannamei new variety mitochondrial control region microsatellite
英文摘要Selective breeding of the Pacific white shrimp Litopenaeus vannamei during the last decade has produced new varieties exhibiting high growth rates and disease resistance. However, the identification of new varieties of shrimps from their phenotypic characters is difficult. This study introduces a new approach for identifying varieties of shrimps using molecular markers of microsatellites and mitochondrial control region sequences. The method was employed to identify a new selected variety, Kehai No. 1 (KH-1), from three representative stocks (control group): Zhengda; Tongwei; and a stock collected from Fujian Province, which is now cultured in mainland China. By pooled genotyping of KH-1 and the control group, five microsatellites showing differences between KH-1 and the control group were screened out. Individual genotyping data confirmed the results from pooled genotyping. The genotyping data for the five microsatellites were applied to the assignment analysis of the KH-1 group and the control group using the partial Bayesian assignment method in GENECLASS2. By sequencing the mitochondrial control regions of individuals from the KH-1 and control group, four haplotypes were observed in the KH-1 group, whereas 14 haplotypes were obtained in the control group. By combining the microsatellite assignment analysis with mitochondrial control region analysis, the average accuracy of identification of individuals in the KH-1 group and control group reached 89%. The five selected microsatellite loci and mitochondrial control region sequences were highly polymorphic and could be used to distinguish new selected varieties of L. vannamei from other populations cultured in China.
收录类别SCI
语种英语
WOS记录号WOS:000351156200001
公开日期2015-06-16
源URL[http://ir.qdio.ac.cn/handle/337002/25424]  
专题海洋研究所_实验海洋生物学重点实验室
作者单位1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
3.Hainan Nanjiang Marine Biotech Co Ltd, Sanya 572536, Peoples R China
4.Northwest Agr & Forestry Univ, Yangling 712100, Peoples R China
推荐引用方式
GB/T 7714
Yu Yang,Zhang Xiaojun,Liu Jingwen,et al. Molecular markers for identifying a new selected variety of Pacific white shrimp Litopenaeus vannamei[J]. CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,2015,33(1):1-10.
APA Yu Yang.,Zhang Xiaojun.,Liu Jingwen.,Li Fuhua.,Huang Hao.,...&Xiang Jianhai.(2015).Molecular markers for identifying a new selected variety of Pacific white shrimp Litopenaeus vannamei.CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,33(1),1-10.
MLA Yu Yang,et al."Molecular markers for identifying a new selected variety of Pacific white shrimp Litopenaeus vannamei".CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY 33.1(2015):1-10.

入库方式: OAI收割

来源:海洋研究所

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