Molecular markers for identifying a new selected variety of Pacific white shrimp Litopenaeus vannamei
文献类型：期刊论文
| 作者 | Yu Yang1,2 ; Zhang Xiaojun1; Liu Jingwen1,2; Li Fuhua1; Huang Hao3; Li Yijun3; Liu Xiaolin4; Xiang Jianhai1
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| 刊名 | CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY
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| 出版日期 | 2015 |
| 卷号 | 33期号:1页码:1-10 |
| 关键词 | molecular identification Litopenaeus vannamei new variety mitochondrial control region microsatellite |
| 英文摘要 | Selective breeding of the Pacific white shrimp Litopenaeus vannamei during the last decade has produced new varieties exhibiting high growth rates and disease resistance. However, the identification of new varieties of shrimps from their phenotypic characters is difficult. This study introduces a new approach for identifying varieties of shrimps using molecular markers of microsatellites and mitochondrial control region sequences. The method was employed to identify a new selected variety, Kehai No. 1 (KH-1), from three representative stocks (control group): Zhengda; Tongwei; and a stock collected from Fujian Province, which is now cultured in mainland China. By pooled genotyping of KH-1 and the control group, five microsatellites showing differences between KH-1 and the control group were screened out. Individual genotyping data confirmed the results from pooled genotyping. The genotyping data for the five microsatellites were applied to the assignment analysis of the KH-1 group and the control group using the partial Bayesian assignment method in GENECLASS2. By sequencing the mitochondrial control regions of individuals from the KH-1 and control group, four haplotypes were observed in the KH-1 group, whereas 14 haplotypes were obtained in the control group. By combining the microsatellite assignment analysis with mitochondrial control region analysis, the average accuracy of identification of individuals in the KH-1 group and control group reached 89%. The five selected microsatellite loci and mitochondrial control region sequences were highly polymorphic and could be used to distinguish new selected varieties of L. vannamei from other populations cultured in China. |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000351156200001 |
| 公开日期 | 2015-06-16 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/25424]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Hainan Nanjiang Marine Biotech Co Ltd, Sanya 572536, Peoples R China 4.Northwest Agr & Forestry Univ, Yangling 712100, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yu Yang,Zhang Xiaojun,Liu Jingwen,et al. Molecular markers for identifying a new selected variety of Pacific white shrimp Litopenaeus vannamei[J]. CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,2015,33(1):1-10. |
| APA | Yu Yang.,Zhang Xiaojun.,Liu Jingwen.,Li Fuhua.,Huang Hao.,...&Xiang Jianhai.(2015).Molecular markers for identifying a new selected variety of Pacific white shrimp Litopenaeus vannamei.CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,33(1),1-10. |
| MLA | Yu Yang,et al."Molecular markers for identifying a new selected variety of Pacific white shrimp Litopenaeus vannamei".CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY 33.1(2015):1-10. |
入库方式： OAI收割
来源：海洋研究所
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