Molecular characterization of the cathepsin B of turbot (Scophthalmus maximus)
文献类型：期刊论文
| 作者 | Zhou, Ze-jun1,2 ; Qiu, Reng1,2,3; Zhang, Jian1
|
| 刊名 | FISH PHYSIOLOGY AND BIOCHEMISTRY
 |
| 出版日期 | 2015-04-01 |
| 卷号 | 41期号:2页码:473-483 |
| 关键词 | Cathepsin B Scophthalmus maximus Expression Cysteine protease |
| 英文摘要 | Cathepsin B is an enzymatic protein belonging to the peptidase C1 family. It is involved in diverse physiological and pathological functions that include immune response. In this study, we identified and characterized a cathepsin B homolog (SmCatB) from turbot (Scophthalmus maximus). SmCatB is composed of 330 amino acid residues and possesses typical domain architecture of cathepsin B, which contains a propeptide region and a cysteine protease domain, and the latter processes four conserved residues (Q101, C107, H277, and N297) in the active site. SmCatB shares 80.6-87.6 % overall sequence identities with the cathepsin B of a number of teleost. SmCatB expression was detected in a wide range of tissues and upregulated by bacterial infection in a time-dependent manner. Recombinant SmCatB (rSmCatB-WT) purified from Escherichia coli exhibited apparent protease activity, which was optimal at 50 degrees C and pH 5.5. Compared to rSmCatB-WT, the mutant proteins rSmCatB-C107S, rSmCatB-H277A, and rSmCatB-N297A, which bear C107S, H277A, and N297A mutations, respectively, were significantly reduced in protease activity, with the highest reduction observed with rSmCatB-N297A. These results indicate that SmCatB is a bioactive protease that depends on the conserved structural features and that SmCatB is involved in pathogen-induced immune response. |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000351506900013 |
| 公开日期 | 2015-06-16 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/25441]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Nanyang Normal Univ, China UK NYNU RRes Joint Lab Insect Biol, Nanyang 473061, Henan, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhou, Ze-jun,Qiu, Reng,Zhang, Jian. Molecular characterization of the cathepsin B of turbot (Scophthalmus maximus)[J]. FISH PHYSIOLOGY AND BIOCHEMISTRY,2015,41(2):473-483. |
| APA | Zhou, Ze-jun,Qiu, Reng,&Zhang, Jian.(2015).Molecular characterization of the cathepsin B of turbot (Scophthalmus maximus).FISH PHYSIOLOGY AND BIOCHEMISTRY,41(2),473-483. |
| MLA | Zhou, Ze-jun,et al."Molecular characterization of the cathepsin B of turbot (Scophthalmus maximus)".FISH PHYSIOLOGY AND BIOCHEMISTRY 41.2(2015):473-483. |
入库方式： OAI收割
来源：海洋研究所
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