Structural Analysis and Anti-Complement Activity of Polysaccharides from Kjellmaniella crsaaifolia
文献类型：期刊论文
| 作者 | Zhang, Wenjing1,2 ; Jin, Weihua1; Sun, Delin3; Zhao, Luyu3; Wang, Jing1,4; Duan, Delin1; Zhang, Quanbin1
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| 刊名 | MARINE DRUGS
 |
| 出版日期 | 2015-03-01 |
| 卷号 | 13期号:3页码:1360-1374 |
| 英文摘要 | Two polysaccharides, named KCA and KCW, were extracted from Kjellmaniella crassifolia using dilute hydrochloric acid and water, respectively. Composition analysis showed that these polysaccharides predominantly consisted of fucose, with galactose, mannose and glucuronic acid as minor components. After degradation and partial desulfation, electrospray ionization mass spectrometry (ESI-MS) was performed, which showed that the polysaccharides consisted of sulfated fucooligosaccharides, sulfated galactofucooligosaccharides and methyl glycosides of mono-sulfated/multi-sulfated fucooligosaccharides. The structures of the oligomeric fragments were further characterized by electrospray ionization collision-induced dissociation tandem mass spectrometry (ESI-CID-MS2 and ESI-CID-MS3). Moreover, the activity of KCA and KCW against the hemolytic activity of both the classical and alternative complement pathways was determined. The activity of KCA was found to be similar to KCW, suggesting that the method of extraction did not influence the activity. In addition, the degraded polysaccharides (DKCA and DKCW) displayed lower activity levels than the crude polysaccharides (KCA and KCW), indicating that molecular weight had an effect on activity. Moreover, the desulfated fractions (ds-DKCA and ds-DKCW) showed less or no activity, which confirmed that sulfate was important for activity. In conclusion, polysaccharides from K. crassifolia may be good candidates for the treatment of diseases involving the complement pathway. |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000351930500015 |
| 公开日期 | 2015-06-16 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/25447]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 海洋研究所_海洋生物技术研发中心 |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 2.Univ Chinese Acad Sci, Coll Earth Sci, Beijing 100049, Peoples R China 3.Heze Juxinyuan Food Co Ltd, Heze 274400, Peoples R China 4.Chinese Acad Sci, Nantong Branch, Inst Oceanol, Nantong 226006, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Wenjing,Jin, Weihua,Sun, Delin,et al. Structural Analysis and Anti-Complement Activity of Polysaccharides from Kjellmaniella crsaaifolia[J]. MARINE DRUGS,2015,13(3):1360-1374. |
| APA | Zhang, Wenjing.,Jin, Weihua.,Sun, Delin.,Zhao, Luyu.,Wang, Jing.,...&Zhang, Quanbin.(2015).Structural Analysis and Anti-Complement Activity of Polysaccharides from Kjellmaniella crsaaifolia.MARINE DRUGS,13(3),1360-1374. |
| MLA | Zhang, Wenjing,et al."Structural Analysis and Anti-Complement Activity of Polysaccharides from Kjellmaniella crsaaifolia".MARINE DRUGS 13.3(2015):1360-1374. |
入库方式： OAI收割
来源：海洋研究所
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