Nuclei fluorescence microscopic observation on early embryonic development of mitogynogenetic diploid induced by hydrostatic pressure treatment in olive flounder (Paralichthys olivaceus)
文献类型：期刊论文
| 作者 | Lin, Zhengmei1; Zhu, Xiangping1; You, Feng2; Wu, Zhihao2; Cao, Yuanshui3 |
| 刊名 | THERIOGENOLOGY
 |
| 出版日期 | 2015-05-01 |
| 卷号 | 83期号:8页码:1310-1320 |
| 关键词 | Olive flounder (Paralichthys olivaceus) Mitogynogenetic diploid Fluorescent microscopic observation Hydrostatic pressure |
| 英文摘要 | Sperm genetic material of olive flounder (Paralichthys olivaceus) was inactivated by ultraviolet irradiation. The nuclear phase changes during early embryonic development of diploid, haploid, and mitogynogenetic diploid induced by hydrostatic pressure treatment were observed under fluorescent microscope with 4',6-diamidino-2-phenylindole staining. The parameters of hydrostatic pressure treatment were 600 kg/cm(2) for 6 minutes at prometaphase stage. The data showed that developmental timing sequence of diploid and haploid fertilized eggs was similar. The cell cycle was about 48 minutes, including interphase (about 21 minutes), prophase (about 3 minutes), prometaphase (about 6 minutes), metaphase (about 6 minutes), anaphase (around 9 minutes), and telophase (about 3 minutes). After entering the fertilized egg, ultraviolet-inactivated sperm formed a male pronucleus and became a dense chromatin body in the cytoplasm. Dense chromatin body did not participate in nuclear division and unchanged all the time. For hydrostatic pressure-treated embryos, the first nuclear division and cytokinesis after treatment proceeded normally after about 15 minutes recovery. During the second mitosis, having undergone interphase, prophase, and prometaphase stage, chromosomes began to slowly spread around and scattered in the cell but not entered into metaphase and anaphase. The second nuclear division and cytokinesis was inhibited. The occurrence frequency of developmentally delayed embryos also showed that the second cleavage of about 80% treated eggs was inhibited. The inhibition of the second cleavage resulted to chromosome set doubling. So chromosome set doubling for mitogynogenetic flounder diploid induced by hydrostatic pressure treatment, performed at prometaphase stage, was mainly due to inhibition of the second mitosis rather than the first one. (C) 2015 Elsevier Inc. All rights reserved. |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000352175300008 |
| 公开日期 | 2015-06-16 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/25451]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 作者单位 | 1.Qingdao Agr Univ, Anim Sci & Technol Dept, Qingdao, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, Qingdao, Peoples R China 3.Shenghang Aquat Sci & Technol Co Ltd, Weihai, Peoples R China |
| 推荐引用方式 GB/T 7714 | Lin, Zhengmei,Zhu, Xiangping,You, Feng,et al. Nuclei fluorescence microscopic observation on early embryonic development of mitogynogenetic diploid induced by hydrostatic pressure treatment in olive flounder (Paralichthys olivaceus)[J]. THERIOGENOLOGY,2015,83(8):1310-1320. |
| APA | Lin, Zhengmei,Zhu, Xiangping,You, Feng,Wu, Zhihao,&Cao, Yuanshui.(2015).Nuclei fluorescence microscopic observation on early embryonic development of mitogynogenetic diploid induced by hydrostatic pressure treatment in olive flounder (Paralichthys olivaceus).THERIOGENOLOGY,83(8),1310-1320. |
| MLA | Lin, Zhengmei,et al."Nuclei fluorescence microscopic observation on early embryonic development of mitogynogenetic diploid induced by hydrostatic pressure treatment in olive flounder (Paralichthys olivaceus)".THERIOGENOLOGY 83.8(2015):1310-1320. |
入库方式： OAI收割
来源：海洋研究所
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