Function and Regulation Domains of a Newly Isolated Putative beta-Actin Promoter from Pacific White Shrimp
文献类型：期刊论文
| 作者 | Shi, Yingli1,2,3 ; Soderlund, Marcus1; Xiang, Jianhai2; Lu, Yuanan1
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| 刊名 | PLOS ONE
 |
| 出版日期 | 2015-04-02 |
| 卷号 | 10期号:4 |
| 英文摘要 | Current development of transgenic shrimp research has been hampered due to the lack of the suitable promoters and efficient transfection methods for crustaceans. A 1642 bp sequence, containing 5'-upstream sequence, exon 1, intron 1 and partial exon 2, which is responsible for transcriptional initiation of the newly reported shrimp beta-actin (actinT1), has been isolated from the Pacific white shrimp (Litopenaeus vannamei) and named as SbaP. To determine its function and potential application in marine biotechnology, the sequence and functional domains were examined by constitutive expression of the luciferase reporter gene. We have identified 5' regions that play a central role in the expression of the mu-actin gene. The proximal promoter (-1642/-1325) contains two highly conserved transcriptional sites, CCAAT box and CArG motif. Two negative (-1140/-924, -222/-21) and one positive (-810/-425) regulatory elements have been identified in intron1. Transient transfection assay with a construct containing proximal promoter and enhancer (SbaP Delta-222/+1 Delta-1325/-924) regions of the shrimp beta-actin coupled with luciferase and EGFP (enhanced green fluorescent protein) showed that the promoter was not only functional in sf21 cells, but promoter activity was more than 8-fold higher than a viral-origin promoter (ie1, white spot syndrome virus immediate early gene promoter). Furthermore, SbaP Delta-222/+1 Delta-1325/924 drove a successful expression of luciferase injection assay in vivo injection and also showed higher promoter activity than the ie1 promoter, suggesting that the expression vectors constructed with SbaP Delta-222/+1 Delta-1325/-924 have important potential in gene transfer studies for shrimp and other crustacean species. |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000352139000090 |
| 公开日期 | 2015-06-16 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/25457]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 作者单位 | 1.Univ Hawaii Manoa, Dept Publ Hlth Sci, Honolulu, HI 96822 USA 2.Chinese Acad Sci, Key Lab Expt Marine Biol, Inst Oceanol, Qingdao, Shandong, Peoples R China 3.Univ Chinese Acad Sci, Beijing, Peoples R China |
| 推荐引用方式 GB/T 7714 | Shi, Yingli,Soderlund, Marcus,Xiang, Jianhai,et al. Function and Regulation Domains of a Newly Isolated Putative beta-Actin Promoter from Pacific White Shrimp[J]. PLOS ONE,2015,10(4). |
| APA | Shi, Yingli,Soderlund, Marcus,Xiang, Jianhai,&Lu, Yuanan.(2015).Function and Regulation Domains of a Newly Isolated Putative beta-Actin Promoter from Pacific White Shrimp.PLOS ONE,10(4). |
| MLA | Shi, Yingli,et al."Function and Regulation Domains of a Newly Isolated Putative beta-Actin Promoter from Pacific White Shrimp".PLOS ONE 10.4(2015). |
入库方式： OAI收割
来源：海洋研究所
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