Pseudomonas fluorescens: Iron-responsive proteins and their involvement in host infection
文献类型：期刊论文
| 作者 | Sun, Yuan-yuan1,2 ; Sun, Li1
|
| 刊名 | VETERINARY MICROBIOLOGY
 |
| 出版日期 | 2015-04-17 |
| 卷号 | 176期号:3-4页码:309-320 |
| 关键词 | Pseudomonas fluorescens Iron Proteomic Virulence Immunoprotection |
| 英文摘要 | For pathogenic bacteria, the ability to acquire iron is vital to survival in the host. In consequence, many genes involved in iron acquisition are associated with bacterial virulence. Pseudomonas fluorescens is a bacterial pathogen to a variety of farmed fish. However, the global regulatory function of iron in pathogenic P. fluorescens is essentially unknown. In this study, in order to identify proteins affected by iron condition at the expression level, we performed proteomic analysis to compare the global protein profiles of P. fluorescens strain TSS, a fish pathogen, cultured under iron-replete and iron-deplete conditions. Twenty-two differentially expressed proteins were identified, most of which were confirmed to be regulated by iron at the mRNA level. To investigate their potential involvement in virulence, the genes encoding four of the 22 proteins, i.e. HemO (heme oxygenase), PspB (serine protease), Sod (superoxide dismutase), and TfeR (TonB-dependent outermembrane ferric enterobactin receptor), were knocked out, and the pathogenicity of the mutants was examined in a model of turbot (Scophthalmus maximus). The results showed that compared to the wild type, the hemO, pspB, and tfeR knockouts were significantly impaired in the ability to survive in host serum, to invade host tissues, and to cause host mortality. Immunization of turbot with recombinant TfeR (rTfeR) and PspB induced production of specific serum antibodies and significant protections against lethal TSS challenge. Further analysis showed that rTfeR antibodies recognized and bound to TSS, and that treatment of TSS with rTfeR antibodies significantly impaired the infectivity of TSS to fish cells. Taken together, these results indicate for the first time that in pathogenic P. fluorescens, iron affects the expression of a large number of proteins including those that are involved in host infection. (C) 2015 Elsevier B.V. All rights reserved. |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000352047600012 |
| 公开日期 | 2015-06-16 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/25461]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Sun, Yuan-yuan,Sun, Li. Pseudomonas fluorescens: Iron-responsive proteins and their involvement in host infection[J]. VETERINARY MICROBIOLOGY,2015,176(3-4):309-320. |
| APA | Sun, Yuan-yuan,&Sun, Li.(2015).Pseudomonas fluorescens: Iron-responsive proteins and their involvement in host infection.VETERINARY MICROBIOLOGY,176(3-4),309-320. |
| MLA | Sun, Yuan-yuan,et al."Pseudomonas fluorescens: Iron-responsive proteins and their involvement in host infection".VETERINARY MICROBIOLOGY 176.3-4(2015):309-320. |
入库方式： OAI收割
来源：海洋研究所
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