Cell penetrating peptide can transport dsRNA into microalgae with thin cell walls
文献类型：期刊论文
| 作者 | Wei, Yifan1,2; Niu, Jianfeng1; Huan, Li1,2 ; Huang, Aiyou1; He, Linwen1; Wang, Guangce1
|
| 刊名 | ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS
 |
| 出版日期 | 2015-03-01 |
| 卷号 | 8页码:135-139 |
| 关键词 | CPPs Microalgae Cell wall Photosynthesis |
| 英文摘要 | Cell penetrating peptides (CPPs) are effective molecular tools that can deliver various exogenous cargoes into different types of cells. However, no study to date has evaluated the use of CPPs to import nucleic acid substances into microalgae, which would be advantageous for numerous research and commercial applications. In this study, we tested the ability of nona-(L)-arginine (R9) to conduct CPP-mediated double-stranded RNA (dsRNA) translocations into four microalgal species (Chlamydomonas reinhardtii, Chlorella vulgaris, Phaeodactylum tricornutum, and Dunaliella salina). R9 successfully transported dsRNA into D. salina, whereas dsRNA internalizations were not detected in the other three algal species. Calcofluor white staining showed that the cell wall structure of D. salina differs from that of the other three species. These results indicate that the efficiency of CPP-mediated dsRNA transductions is closely related to algal cell wall structures, which vary in their ability to block entry of the CPP/ dsRNA complex. (C) 2015 Elsevier B.V. All rights reserved. |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000352274800020 |
| 公开日期 | 2015-06-16 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/25462]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 2.Univ Chinese Acad Sci, Coll Earth Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wei, Yifan,Niu, Jianfeng,Huan, Li,et al. Cell penetrating peptide can transport dsRNA into microalgae with thin cell walls[J]. ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS,2015,8:135-139. |
| APA | Wei, Yifan,Niu, Jianfeng,Huan, Li,Huang, Aiyou,He, Linwen,&Wang, Guangce.(2015).Cell penetrating peptide can transport dsRNA into microalgae with thin cell walls.ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS,8,135-139. |
| MLA | Wei, Yifan,et al."Cell penetrating peptide can transport dsRNA into microalgae with thin cell walls".ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS 8(2015):135-139. |
入库方式： OAI收割
来源：海洋研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。