Edwardsiella tarda evades serum killing by preventing complement activation via the alternative pathway
文献类型：期刊论文
| 作者 | Li, Mo-fei1,2 ; Sun, Li1; Li, Jun3
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| 刊名 | FISH & SHELLFISH IMMUNOLOGY
 |
| 出版日期 | 2015-04-01 |
| 卷号 | 43期号:2页码:325-329 |
| 关键词 | Serum resistance Immune evasion Virulence Bacteria |
| 英文摘要 | Edwardsiella tarda is a Gram-negative bacterium with a broad host range that includes a wide variety of farmed fish as well as humans. E. tarda has long been known to be able to survive in host serum, but the relevant mechanism is unclear. In this study, we investigated the fundamental question, i.e. whether E. tarda activated serum complement or not. We found that (i) when incubated with flounder serum, E. tarda exhibited a high survival rate (87.6%), which was slightly but significantly reduced in the presence of Mg2+; (ii) E. tarda-incubated serum possessed strong hemolytic activity and bactericidal activity, (iii) compared to the serum incubated with a complement-sensitive laboratory Escherichia coli strain, E. tarda-incubated serum exhibited much less chemotactic activity, (iv) in contrast to the serum incubated with live E. tarda, the serum incubated with heat-inactivated E. tarda exhibited no apparent hemolytic capacity. Taken together, these results indicate for the first time that E. tarda circumvents serum attack by preventing, to a large extent, complement activation via the alternative pathway, and that heatlabile surface structures likely play an essential role in the complement evasion of E. tarda. (C) 2015 Elsevier Ltd. All rights reserved. |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000352923700004 |
| 公开日期 | 2015-06-16 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/25474]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China 3.Lake Super State Univ, Sch Biol Sci, Sault Ste Marie, MI 49783 USA |
| 推荐引用方式 GB/T 7714 | Li, Mo-fei,Sun, Li,Li, Jun. Edwardsiella tarda evades serum killing by preventing complement activation via the alternative pathway[J]. FISH & SHELLFISH IMMUNOLOGY,2015,43(2):325-329. |
| APA | Li, Mo-fei,Sun, Li,&Li, Jun.(2015).Edwardsiella tarda evades serum killing by preventing complement activation via the alternative pathway.FISH & SHELLFISH IMMUNOLOGY,43(2),325-329. |
| MLA | Li, Mo-fei,et al."Edwardsiella tarda evades serum killing by preventing complement activation via the alternative pathway".FISH & SHELLFISH IMMUNOLOGY 43.2(2015):325-329. |
入库方式： OAI收割
来源：海洋研究所
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