Baseline Survey of Root-Associated Microbes of Taxus chinensis (Pilger) Rehd
文献类型：期刊论文
| 作者 | Zhang, Qian; Liu, Hongwei; Sun, Guiling ; Wilson, Iain W.; Wu, Jianqiang; Hoffman, Angela; Cheng, Junwen; Qiu, Deyou
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| 刊名 | PLOS ONE
 |
| 出版日期 | 2015-03-30 |
| 卷号 | 10期号:3页码:e0123026 |
| 通讯作者 | Qiu,DY (reprint author),Chinese Acad Forestry,Res Inst Forestry,State Key Lab Tree Genet & Breeding,Beijing 100091,Peoples R China. ; peterqdy@aliyun.com |
| 英文摘要 | Taxol (paclitaxel) a diterpenoid is one of the most effective anticancer drugs identified. Biosynthesis of taxol was considered restricted to the Taxus genera until Stierle et al. discovered that an endophytic fungus isolated from Taxus brevifolia could independently synthesize taxol. Little is known about the mechanism of taxol biosynthesis in microbes, but it has been speculated that its biosynthesis may differ from plants. The microbiome from the roots of Taxus chinensis have been extensively investigated with culture-dependent methods to identify taxol synthesizing microbes, but not using culture independent methods., Using bar-coded high-throughput sequencing in combination with a metagenomics approach, we surveyed the microbial diversity and gene composition of the root-associated microbiomefrom Taxus chinensis (Pilger) Rehd. High-throughput amplicon sequencing revealed 187 fungal OTUs which is higher than any previously reported fungal number identified with the culture-dependent method, suggesting that T. chinensis roots harbor novel and diverse fungi. Some operational taxonomic units (OTU) identified were identical to reported microbe strains possessing the ability to synthesis taxol and several genes previously associated with taxol biosynthesis were identified through metagenomics analysis. |
| 学科主题 | Multidisciplinary Sciences |
| 类目[WOS] | Multidisciplinary Sciences |
| 研究领域[WOS] | Science & Technology - Other Topics |
| 关键词[WOS] | TAXOL-PRODUCING FUNGUS ; ENDOPHYTIC FUNGI ; BACTERIAL DIVERSITY ; TAXANE PRODUCTION ; X-MEDIA ; PACLITAXEL ; YEW ; BIODIVERSITY ; RHIZOSPHERE ; CULTURES |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000352134700212 |
| 源URL | [http://ir.kib.ac.cn/handle/151853/20572]  |
| 专题 | 昆明植物研究所_资源植物与生物技术所级重点实验室 |
| 推荐引用方式 GB/T 7714 | Zhang, Qian,Liu, Hongwei,Sun, Guiling,et al. Baseline Survey of Root-Associated Microbes of Taxus chinensis (Pilger) Rehd[J]. PLOS ONE,2015,10(3):e0123026. |
| APA | Zhang, Qian.,Liu, Hongwei.,Sun, Guiling.,Wilson, Iain W..,Wu, Jianqiang.,...&Qiu, Deyou.(2015).Baseline Survey of Root-Associated Microbes of Taxus chinensis (Pilger) Rehd.PLOS ONE,10(3),e0123026. |
| MLA | Zhang, Qian,et al."Baseline Survey of Root-Associated Microbes of Taxus chinensis (Pilger) Rehd".PLOS ONE 10.3(2015):e0123026. |
入库方式： OAI收割
来源：昆明植物研究所
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