Purification and Characterization of Alkaline Pectin Lyase from a Newly Isolated Bacillus clausii and Its Application in Elicitation of Plant Disease Resistance
文献类型：期刊论文
| 作者 | Li, Zuming ; Bai, Zhihui ; Zhang, Baoguo ; Li, Baojv ; Jin, Bo ; Zhang, Michael ; Lin, Francis ; Zhang, Hongxun |
| 刊名 | APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
 |
| 出版日期 | 2012 |
| 卷号 | 167期号:8页码:2241－2256 |
| 关键词 | Bacillus clausii Alkaline pectin lyase Sugar beet pulp Mass spectrometric sequencing Plant disease resistance |
| ISSN号 | 0273-2289 |
| 中文摘要 | Alkaline pectin lyase (PNL) shows potential as a biological control agent against several plant diseases. We isolated and characterized a new Bacillus clausii strain that can produce 4,180 U/g of PNL using sugar beet pulp as a carbon source and inducer. The PNL was purified to apparent homogeneity using ultrafiltration, ammonium sulfate fractionation, DEAE Sepharose Fast Flow, and Sephadex G-75 gel filtration. The purified PNL was found to be a monomeric protein with a molecular weight of 35 kDa, as determined by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). It demonstrated optimal activity with K (m) of 0.87 mg/ml at pH 10.0 and 60 A degrees C. The enzyme is stable in the pH range of 8.0-10.0 and temperature a parts per thousand currency sign40 A degrees C. Ca2+ was found to stimulate the enzymatic activity of the PNL by up to 410 %. Mass spectrometric results gave 38 % match coverage with pectate lyase from B. clausii KSM-K16 (gi|56961845). The PNL was found to elicit disease resistance in cucumber seedlings, suggesting that it may have applications in biocontrol and sustainable agriculture. |
| WOS记录号 | WOS:000307537500009 |
| 公开日期 | 2014-11-05 |
| 源URL | [http://ir.rcees.ac.cn/handle/311016/7920]  |
| 专题 | 生态环境研究中心_中国科学院环境生物技术重点实验室 |
| 推荐引用方式 GB/T 7714 | Li, Zuming,Bai, Zhihui,Zhang, Baoguo,et al. Purification and Characterization of Alkaline Pectin Lyase from a Newly Isolated Bacillus clausii and Its Application in Elicitation of Plant Disease Resistance[J]. APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY,2012,167(8):2241－2256. |
| APA | Li, Zuming.,Bai, Zhihui.,Zhang, Baoguo.,Li, Baojv.,Jin, Bo.,...&Zhang, Hongxun.(2012).Purification and Characterization of Alkaline Pectin Lyase from a Newly Isolated Bacillus clausii and Its Application in Elicitation of Plant Disease Resistance.APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY,167(8),2241－2256. |
| MLA | Li, Zuming,et al."Purification and Characterization of Alkaline Pectin Lyase from a Newly Isolated Bacillus clausii and Its Application in Elicitation of Plant Disease Resistance".APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY 167.8(2012):2241－2256. |
入库方式： OAI收割
来源：生态环境研究中心
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