Pyrosequencing reveals the dominance of methylotrophic methanogenesis in a coal bed methane reservoir associated with Eastern Ordos Basin in China
文献类型：期刊论文
| 作者 | Guo, Hongguang ; Liu, Ruyin ; Yu, Zhisheng ; Zhang, Hongxun ; Yun, Juanli ; Li, Yaoming ; Liu, Xiu ; Pan, Jiangang |
| 刊名 | INTERNATIONAL JOURNAL OF COAL GEOLOGY
 |
| 出版日期 | 2012 |
| 卷号 | 93页码:56－61 |
| 关键词 | Coal bed methane Pyrosequencing Methanogen Methanogenesis Ordos Basin |
| ISSN号 | 0166-5162 |
| 中文摘要 | It is generally believed that biogenic coal bed methane (CBM) is an end product of coal biodegradation by methanogenic archaea and syntrophic bacteria. In this work, the archaeal and bacterial communities of CBM reservoir associated with Ordos Basin in China were investigated using 454 pyrosequencing. Sampling produced water, coal and rock in the reservoir, a total of 46,598 sequence reads were obtained. All archaea were methanogens with the genus Methanolobus predominating. The genus consisted of 81.18% of pyrosequencing reads in water sample and >99% in coal and rock samples. Although the phylum Proteobacteria was the main component of all samples, bacterial communities in coal and rock samples were similar at the genus level, which were distinctly separated with water sample. The results strongly suggested that methylotrophic methanogenesis governed the biogenic CBM formation. The separation of microbial communities between water and coal, rock samples should be considered when investigating the process of coal biodegradation and the generation of new biogenic CBM. (C) 2012 Elsevier B.V. All rights reserved. |
| WOS记录号 | WOS:000302432400006 |
| 公开日期 | 2014-11-05 |
| 源URL | [http://ir.rcees.ac.cn/handle/311016/7921]  |
| 专题 | 生态环境研究中心_中国科学院环境生物技术重点实验室 |
| 推荐引用方式 GB/T 7714 | Guo, Hongguang,Liu, Ruyin,Yu, Zhisheng,et al. Pyrosequencing reveals the dominance of methylotrophic methanogenesis in a coal bed methane reservoir associated with Eastern Ordos Basin in China[J]. INTERNATIONAL JOURNAL OF COAL GEOLOGY,2012,93:56－61. |
| APA | Guo, Hongguang.,Liu, Ruyin.,Yu, Zhisheng.,Zhang, Hongxun.,Yun, Juanli.,...&Pan, Jiangang.(2012).Pyrosequencing reveals the dominance of methylotrophic methanogenesis in a coal bed methane reservoir associated with Eastern Ordos Basin in China.INTERNATIONAL JOURNAL OF COAL GEOLOGY,93,56－61. |
| MLA | Guo, Hongguang,et al."Pyrosequencing reveals the dominance of methylotrophic methanogenesis in a coal bed methane reservoir associated with Eastern Ordos Basin in China".INTERNATIONAL JOURNAL OF COAL GEOLOGY 93(2012):56－61. |
入库方式： OAI收割
来源：生态环境研究中心
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