Multivariate analysis linkage of phyllospheric microbial community of transgenic cotton from SGK321 to Cry1Ac: A temporal expression dynamics
文献类型：期刊论文
| 作者 | Pan, Jiangang ; Cui, Mengmeng ; Hu, Qing ; Ma, Anzhou ; Bai, Zhihui ; Yang, Dianlin ; Zhang, Hongxun ; Guo, Hongguang ; Qi, Hongyan |
| 刊名 | Microbiology
 |
| 出版日期 | 2012 |
| 卷号 | 6期号:25页码:5371-5382 |
| 关键词 | Bt cotton microbial diversity 16S rRNA 18S rRNA phyllosphere |
| ISSN号 | 1996-0808 |
| 中文摘要 | The effects of transgenic plant on ecological and food biosafety have attracted considerable attention recently due to the exploding number of commercialization genetic modified plants. Here, we examined the changes of bacterial and fungal community compositions in the phyllosphere of Cry1Ac Bacillus thuringiensis (Bt) cotton SGK321 by molecular fingerprint method at four major growth stages. The phyllospheric bacteria diversity of SGK321 was decreased at budding stage only, indicated by Shannon index. A slight increase of diversity and abundance of fungal community could be detected at different sampling stage except the seeding stage. Fungal community showed a tendency to be affiliated with the expression of Cry1Ac protein which was detected by enzyme-linked immunosorbent assay (ELISA) method at both the seeding and budding stages. Results suggested that, compared to bacteria, fungi in phyllosphere of transgenic cotton SGK321 might be more susceptible to Cry1Ac protein, which includes Cochliobolus sp., Setosphaeria sp., Blakeslea trispora, Cryptococcus neoformans and Cryptococcus sp. Furthermore, the seasonal and temporal parameters during the growth stages were proved to be the most important factor relating with the shift of phyllospheric microorganism communities. |
| WOS记录号 | WOS:000307506000012 |
| 公开日期 | 2014-11-28 |
| 源URL | [http://ir.rcees.ac.cn/handle/311016/8193]  |
| 专题 | 生态环境研究中心_中国科学院环境生物技术重点实验室 |
| 推荐引用方式 GB/T 7714 | Pan, Jiangang,Cui, Mengmeng,Hu, Qing,et al. Multivariate analysis linkage of phyllospheric microbial community of transgenic cotton from SGK321 to Cry1Ac: A temporal expression dynamics[J]. Microbiology,2012,6(25):5371-5382. |
| APA | Pan, Jiangang.,Cui, Mengmeng.,Hu, Qing.,Ma, Anzhou.,Bai, Zhihui.,...&Qi, Hongyan.(2012).Multivariate analysis linkage of phyllospheric microbial community of transgenic cotton from SGK321 to Cry1Ac: A temporal expression dynamics.Microbiology,6(25),5371-5382. |
| MLA | Pan, Jiangang,et al."Multivariate analysis linkage of phyllospheric microbial community of transgenic cotton from SGK321 to Cry1Ac: A temporal expression dynamics".Microbiology 6.25(2012):5371-5382. |
入库方式： OAI收割
来源：生态环境研究中心
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