Efficient production of succinic acid from macroalgae hydrolysate by metabolically engineered Escherichia coli
文献类型：期刊论文
| 作者 | Bai, Bing1,2; Zhou, Jie-min1,2; Yang, Mao-hua1; Liu, Yi-lan1,2; Xu, Xiao-hui1,2; Xing, Jian-min1 |
| 刊名 | BIORESOURCE TECHNOLOGY
 |
| 出版日期 | 2015-06-01 |
| 卷号 | 185页码:56-61 |
| 关键词 | Escherichia coli Laminaria japonica Macroalgae Mannitol Succinic acid |
| ISSN号 | 0960-8524 |
| 英文摘要 | In this study, microbial production of succinic acid from macroalgae (i.e., Laminaria japonica) was investigated for the first time. The engineered Escherichia coli BS002 exhibited higher molar yield of succinic acid on mannitol (1.39 +/- 0.01 mol/mol) than glucose (1.01 +/- 0.05 mol/mol). After pretreatment and enzymatic hydrolysis, L. japonica hydrolysate was mainly glucose (10.31 +/- 0.32 g/L) and mannitol (10.12 +/- 0.17 g/L), which was used as the substrate for succinic acid fermentation with the recombinant BS002. A final 17.44 +/- 0.54 g/L succinic acid was obtained from the hydrolysate after 72 h dual-phase fermentation. The yield was as high as 1.24 +/- 0.08 mol/mol total sugar, which reached 73% of the maximum theoretical yield. The results demonstrate that macroalgae biomass represents a novelty and economical alternative feedstock for biochemicals production. (C) 2015 Elsevier Ltd. All rights reserved. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine ; Technology |
| 类目[WOS] | Agricultural Engineering ; Biotechnology & Applied Microbiology ; Energy & Fuels |
| 研究领域[WOS] | Agriculture ; Biotechnology & Applied Microbiology ; Energy & Fuels |
| 关键词[WOS] | CONSERVING PATHWAYS ; SEAWEED HYDROLYSATE ; LAMINARIA-JAPONICA ; NADH/NAD(+) RATIO ; CARBON-SOURCES ; NAD(H) POOL ; EVOLUTION ; PLATFORM ; SACCHARIFICATION ; OVEREXPRESSION |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000353190500009 |
| 公开日期 | 2015-07-08 |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/16739]  |
| 专题 | 过程工程研究所_生化工程国家重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100190, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Bai, Bing,Zhou, Jie-min,Yang, Mao-hua,et al. Efficient production of succinic acid from macroalgae hydrolysate by metabolically engineered Escherichia coli[J]. BIORESOURCE TECHNOLOGY,2015,185:56-61. |
| APA | Bai, Bing,Zhou, Jie-min,Yang, Mao-hua,Liu, Yi-lan,Xu, Xiao-hui,&Xing, Jian-min.(2015).Efficient production of succinic acid from macroalgae hydrolysate by metabolically engineered Escherichia coli.BIORESOURCE TECHNOLOGY,185,56-61. |
| MLA | Bai, Bing,et al."Efficient production of succinic acid from macroalgae hydrolysate by metabolically engineered Escherichia coli".BIORESOURCE TECHNOLOGY 185(2015):56-61. |
入库方式： OAI收割
来源：过程工程研究所
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