Dual-functional OPH-immobilized polyamide nanofibrous membrane for effective organophosphorus toxic agents protection
文献类型：期刊论文
| 作者 | Yan, Xiao-Yu1,2; Jiang, Yan-Jun1; Zhang, Song-Ping2,3; Gao, Jing1; Zhang, Yu-Fei2 |
| 刊名 | BIOCHEMICAL ENGINEERING JOURNAL
 |
| 出版日期 | 2015-06-01 |
| 卷号 | 98期号:15页码:47-55 |
| ISSN号 | 1369-703X |
| 关键词 | Nanofibrous membrane Immobilized enzymes Biodegradation Particle filtration Enzyme biocatalysis Kinetic parameters |
| 英文摘要 | In both civilian and military domains, the increasing use of highly toxic organophosphates (OPs) has created an urgent demand for developing an effective method that could protect the human body from OP poisoning. In this study, a novel dual-functional protecting material with both OP filtration and degradation functions was designed and fabricated. The filtration function was endowed by the electrospinning polyamide 66 (Nylon 66, PA-66) nanofibrous membrane, while the OP degradation function was based on the immobilization of organophosphorus hydrolase (OPH) on membrane through glutaraldehyde (GA) crosslinking. A systematic study of the relationship between the membrane structure, filtration ability and OP degradation activity revealed that PA-66 nanofibrous enzyme with 20 mu m thickness exhibited perfect uniform morphology, and the particle filtration efficiency was over 99%. The nanofibrous enzyme exhibited a specific activity of 41 U/g; the enzyme activity recovery was 53.3%, and the methyl parathion (MP) degradation efficiency was 40%. Moreover, the nanofibrous enzyme expressed excellent organic solvent stability; approximately 70% of its initial activity was retained after incubation in xylene solvent for 24 h at 25 degrees C. The residual activity was 40% after being stored for 30 d at 25 degrees C After 10 repeated uses, the residual activity of the nanofibrous membrane was 37%. This study demonstrated the application potential of dual-functional PA-66 nanofibrous enzyme in biochemical protection, aimed at various military and civilian applications. (C) 2015 Elsevier B.V. All rights reserved. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine ; Technology |
| 类目[WOS] | Biotechnology & Applied Microbiology ; Engineering, Chemical |
| 研究领域[WOS] | Biotechnology & Applied Microbiology ; Engineering |
| 关键词[WOS] | ELECTROSPUN NANOFIBERS ; ENZYME IMMOBILIZATION ; PSEUDOMONAS-DIMINUTA ; POLYMER NANOFIBERS ; NERVE AGENTS ; HYDROLYSIS ; PHOSPHOTRIESTERASE ; HYDROLASE ; FIBERS ; NANOCOMPOSITES |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000353008000006 |
| 公开日期 | 2015-07-08 |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/16742]  |
| 专题 | 过程工程研究所_生化工程国家重点实验室 |
| 作者单位 | 1.Hebei Univ Technol, Sch Chem Engn & Technol, Tianjin 300130, Peoples R China 2.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100190, Peoples R China 3.Collaborat Innovat Ctr Chem Sci & Engn Tianjin, Tianjin 300072, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yan, Xiao-Yu,Jiang, Yan-Jun,Zhang, Song-Ping,et al. Dual-functional OPH-immobilized polyamide nanofibrous membrane for effective organophosphorus toxic agents protection[J]. BIOCHEMICAL ENGINEERING JOURNAL,2015,98(15):47-55. |
| APA | Yan, Xiao-Yu,Jiang, Yan-Jun,Zhang, Song-Ping,Gao, Jing,&Zhang, Yu-Fei.(2015).Dual-functional OPH-immobilized polyamide nanofibrous membrane for effective organophosphorus toxic agents protection.BIOCHEMICAL ENGINEERING JOURNAL,98(15),47-55. |
| MLA | Yan, Xiao-Yu,et al."Dual-functional OPH-immobilized polyamide nanofibrous membrane for effective organophosphorus toxic agents protection".BIOCHEMICAL ENGINEERING JOURNAL 98.15(2015):47-55. |
入库方式： OAI收割
来源：过程工程研究所
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