Constitutive plasma membrane targeting and microdomain localization of Dok5 studied by single-molecule microscopy
文献类型：期刊论文
| 作者 | Fu Guo ; Zhang Feng ; Cao Li ; Xu ZZ(徐至展) ; Chen Yi-zhang ; Wang Gui-ying ; He Cheng |
| 刊名 | biophys. chem.
 |
| 出版日期 | 2008 |
| 卷号 | 136期号:1页码:13 |
| 关键词 | Dok single-molecule pleckstrin homology domain microdomain diffusion total internal reflection fluorescence microscopy |
| ISSN号 | 0301-4622 |
| 中文摘要 | in the present study, single-molecule fluorescence microscopy was used to examine the characteristics of plasma membrane targeting and microdomain localization of enhanced yellow fluorescent protein (eyfp)-tagged wild-type dok5 and its variants in living chinese hamster ovary (cho) cells. we found that dok5 can target constitutively to the plasma membrane, and the ph domain is essential for this process. furthermore, single-molecule trajectories analysis revealed that dok5 can constitutively partition into microdomain on the plasma membrane. finally, the potential mechanism of microdomain localization of dok5 was discussed. this study provided insights into the characteristics of plasma membrane targeting and microdomain localization of dok5 in living cho cells. (c) 2008 elsevier b.v. all rights reserved. |
| 学科主题 | 激光技术;激光物理与基本理论 |
| 语种 | 英语 |
| WOS记录号 | WOS:000257008000003 |
| 公开日期 | 2009-09-18 |
| 源URL | [http://ir.siom.ac.cn/handle/181231/490]  |
| 专题 | 上海光学精密机械研究所_强场激光物理国家重点实验室 |
| 推荐引用方式 GB/T 7714 | Fu Guo,Zhang Feng,Cao Li,et al. Constitutive plasma membrane targeting and microdomain localization of Dok5 studied by single-molecule microscopy[J]. biophys. chem.,2008,136(1):13, 18. |
| APA | Fu Guo.,Zhang Feng.,Cao Li.,徐至展.,Chen Yi-zhang.,...&He Cheng.(2008).Constitutive plasma membrane targeting and microdomain localization of Dok5 studied by single-molecule microscopy.biophys. chem.,136(1),13. |
| MLA | Fu Guo,et al."Constitutive plasma membrane targeting and microdomain localization of Dok5 studied by single-molecule microscopy".biophys. chem. 136.1(2008):13. |
入库方式： OAI收割
来源：上海光学精密机械研究所
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