In Situ Structural Characterization of a Recombinant Protein in Native Escherichia coli Membranes with Solid-State Magic-Angle-Spinning NMR
文献类型：期刊论文
| 作者 | Fu, Riqiang2; Wang, Xingsheng1; Li, Conggang3; Santiago-Miranda, Adriana N.4; Pielak, Gary J.5; Tian, Fang1 |
| 刊名 | JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
 |
| 出版日期 | 2011-08-17 |
| 卷号 | 133期号:32页码:12370-12373 |
| 产权排序 | 第三 |
| 通讯作者 | 李从刚 |
| 英文摘要 | The feasibility of using solid-state magic-angle-spinning NMR spectroscopy for in situ structural characterization of the LR11 (sorLA) transmembrane domain (TM) in native Escherichia coli membranes is presented. LR11 interacts with the human amyloid precursor protein (APP), a central player in the pathology of Alzheimer's disease. The background signals from E. coli lipids and membrane proteins had only minor effects on the LR11 TM resonances. Approximately 50% of the LR11 TM residues were assigned by using C-13 PARIS data. These assignments allowed comparisons of the secondary structure of the LR11 TM in native membrane environments and commonly used membrane mimics (e.g., micelles). In situ spectroscopy bypasses several obstacles in the preparation of membrane proteins for structural analysis and offers the opportunity to investigate how membrane heterogeneity, bilayer asymmetry, chemical gradients, and macromolecular crowding affect the protein structure. |
| WOS标题词 | Science & Technology ; Physical Sciences |
| 学科主题 | 无线电物理 |
| 类目[WOS] | Chemistry, Multidisciplinary |
| 研究领域[WOS] | Chemistry |
| 关键词[WOS] | AMYLOID PRECURSOR PROTEIN ; RESONANCE ASSIGNMENT ; ALZHEIMER-DISEASE ; SECONDARY STRUCTURE ; PURPLE MEMBRANES ; LIPID-MEMBRANES ; COAT PROTEIN ; C-13 ; BACTERIORHODOPSIN ; SPECTROSCOPY |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000294740000014 |
| 源URL | [http://ir.wipm.ac.cn/handle/112942/1752]  |
| 专题 | 武汉物理与数学研究所_2011年以前论文发表（包括2011年） |
| 作者单位 | 1.Penn State Univ, Dept Biochem & Mol Biol, Hershey, PA 17033 USA 2.Natl High Magnet Field Lab, Tallahassee, FL 32310 USA 3.Chinese Acad Sci, Wuhan Inst Phys & Math, State Key Lab Magnet Resonance & Atom & Mol Phys, Wuhan 430071, Peoples R China 4.Univ Puerto Rico, Dept Chem Engn, Mayaguez, PR 00681 USA 5.Univ N Carolina, Dept Chem, Chapel Hill, NC 27599 USA |
| 推荐引用方式 GB/T 7714 | Fu, Riqiang,Wang, Xingsheng,Li, Conggang,et al. In Situ Structural Characterization of a Recombinant Protein in Native Escherichia coli Membranes with Solid-State Magic-Angle-Spinning NMR[J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY,2011,133(32):12370-12373. |
| APA | Fu, Riqiang,Wang, Xingsheng,Li, Conggang,Santiago-Miranda, Adriana N.,Pielak, Gary J.,&Tian, Fang.(2011).In Situ Structural Characterization of a Recombinant Protein in Native Escherichia coli Membranes with Solid-State Magic-Angle-Spinning NMR.JOURNAL OF THE AMERICAN CHEMICAL SOCIETY,133(32),12370-12373. |
| MLA | Fu, Riqiang,et al."In Situ Structural Characterization of a Recombinant Protein in Native Escherichia coli Membranes with Solid-State Magic-Angle-Spinning NMR".JOURNAL OF THE AMERICAN CHEMICAL SOCIETY 133.32(2011):12370-12373. |
入库方式： OAI收割
来源：武汉物理与数学研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。