Chlorotoxin-modified macromolecular contrast agent for MRI tumor diagnosis
文献类型：期刊论文
| 作者 | Huang, Rongqin1,2,3; Han, Liang1,2,3; Li, Jianfeng1,2,3; Liu, Shuhuan1,2,3; Shao, Kun1,2,3; Kuang, Yuyang1,2,3; Hu, Xing1,2,3; Wang, Xuxia4; Lei, Hao4; Jiang, Chen1,2,3 |
| 刊名 | BIOMATERIALS
 |
| 出版日期 | 2011-08-01 |
| 卷号 | 32期号:22页码:5177-5186 |
| 关键词 | Chlorotoxin DGLs MRI Contrast agent Targeted tumor diagnosis |
| 产权排序 | 第三 |
| 通讯作者 | Chen Jiang |
| 英文摘要 | Clinical diagnosis of cancers using magnetic resonance imaging (MRI) is highly dependent on contrast agents, especially for brain tumors which contain blood-brain barrier (BBB) at the early stage. However, currently mostly used low molecular weight contrast agents such as Gd-DTPA suffer from rapid renal clearance, non-specificity, and low contrast efficiency. The aim of this paper is to investigate the potential of a macromolecular MRI contrast agent based on dendrigraft poly-L-lysines (DGLs), using chlorotoxin (CTX) as a tumor-specific ligand. The contrast agent using CTX-modified conjugate as the main scaffold and Gd-DTPA as the payload was successfully synthesized. The results of fluorescent microscopy showed that the modification of CTX could markedly enhance the cellular uptake in C6 glioma and liver tumor cell lines, but not in normal cell line. Significantly increased accumulation of CTX-modified conjugate within glioma and liver tumor was further demonstrated in tumor-bearing nude mice using in vivo imaging system. The MRI results showed that the signal enhancement of mice treated with CTX-modified contrast reached peak level at 5 min for both glioma and liver tumor, 144.97% +/- 19.54% and 158.69% +/- 12.41%, respectively, significantly higher than that of unmodified counterpart and commercial control. And most importantly, the signal enhancement of CTX-modified contrast agent maintained much longer compared to that of controls, which might be useful for more exact diagnosis for tumors. CTX-modified dendrimer-based conjugate might be applied as an efficient MRI contrast agent for targeted and accurate tumor diagnosis. This finding is especially important for tumors such as brain glioma which is known hard to be diagnosed due to the presence of BBB. (C) 2011 Elsevier Ltd. All rights reserved. |
| WOS标题词 | Science & Technology ; Technology |
| 学科主题 | 波谱分析 |
| 类目[WOS] | Engineering, Biomedical ; Materials Science, Biomaterials |
| 研究领域[WOS] | Engineering ; Materials Science |
| 关键词[WOS] | TARGETED GENE DELIVERY ; MAGNETIC-RESONANCE ; DENDRIMER ; CANCER ; NANOPARTICLES ; THERAPY ; GLIOMAS ; LIGAND ; BRAIN |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000291900700020 |
| 源URL | [http://ir.wipm.ac.cn/handle/112942/1756]  |
| 专题 | 武汉物理与数学研究所_2011年以前论文发表（包括2011年） |
| 作者单位 | 1.Fudan Univ, Dept Pharmaceut, Sch Pharm, Shanghai 201203, Peoples R China 2.PLA, Beijing, Peoples R China 3.Minist Educ, Key Lab Smart Drug Delivery, Beijing, Peoples R China 4.Chinese Acad Sci, Wuhan Inst Phys & Math, State Key Lab Magnet Resonance & Atom & Mol Phys, Wuhan 430071, Hubei, Peoples R China |
| 推荐引用方式 GB/T 7714 | Huang, Rongqin,Han, Liang,Li, Jianfeng,et al. Chlorotoxin-modified macromolecular contrast agent for MRI tumor diagnosis[J]. BIOMATERIALS,2011,32(22):5177-5186. |
| APA | Huang, Rongqin.,Han, Liang.,Li, Jianfeng.,Liu, Shuhuan.,Shao, Kun.,...&Jiang, Chen.(2011).Chlorotoxin-modified macromolecular contrast agent for MRI tumor diagnosis.BIOMATERIALS,32(22),5177-5186. |
| MLA | Huang, Rongqin,et al."Chlorotoxin-modified macromolecular contrast agent for MRI tumor diagnosis".BIOMATERIALS 32.22(2011):5177-5186. |
入库方式： OAI收割
来源：武汉物理与数学研究所
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