Combined Metabonomic and Quantitative Real-Time PCR Analyses Reveal Systems Metabolic Changes of Fusarium graminearum Induced by Tri5 Gene Deletion
文献类型：期刊论文
| 作者 | Chen, Fangfang2; Zhang, Jingtao1,3; Song, Xiushi2; Yang, Jian2; Li, Heping2; Tang, Huiru1; Liao, Yu-Cai2,4,5 |
| 刊名 | JOURNAL OF PROTEOME RESEARCH
 |
| 出版日期 | 2011-05-01 |
| 卷号 | 10期号:5页码:2273-2285 |
| 关键词 | (1)H NMR Tri5 gene metabonomics Fusarium graminearum quantitative real-time PCR |
| 产权排序 | 第二 |
| 通讯作者 | Liao,YC |
| 英文摘要 | Fusarium graminearum (FG) is a serious plant pathogen causing huge losses in global production of wheat and other cereals. Tri5-gene encoded trichodiene synthase is the first key enzyme for biosynthesis of trichothecene mycotoxins in FG. To further our understandings of FG metabolism which is essential for developing novel strategies for controlling FG, we conducted a comprehensive investigation on the metabolic changes caused by Tri5-deletion by comparing metabolic differences between the wild-type FG5035 and an FG strain, Tri5(-), with Tri5 deleted. NMR methods identified more than 50 assigned fungal metabolites. Combined metabonomic and quantitative RT-PCR (qRT-PCR) analyses revealed that Tri5 deletion caused significant and comprehensive metabolic changes for FG apart from mycotoxin biosynthesis. These changes involved both carbon and nitrogen metabolisms including alterations in GABA shunt, TCA cycle, shikimate pathway, and metabolisms of lipids, amino acids, inositol, choline, pyrimidine, and purine. The hexose transporter has also been affected. These findings have shown that Tri5 gene deletion induces widespread changes in FG primary metabolism and demonstrated the combination of NMR-based metabonomics and qRT-PCR analyses as a useful way to understand the systems metabolic changes resulting from a single specific gene knockout in an eukaryotic genome and thus Tri5 gene functions. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 学科主题 | 波谱分析 |
| 类目[WOS] | Biochemical Research Methods |
| 研究领域[WOS] | Biochemistry & Molecular Biology |
| 关键词[WOS] | MULTIVARIATE DATA-ANALYSIS ; SPINNING H-1-NMR SPECTROSCOPY ; HEAD BLIGHT ; TRICHOTHECENE BIOSYNTHESIS ; ARABIDOPSIS-THALIANA ; REDUCED VIRULENCE ; WHEAT ; RESPONSES ; CHINA ; SCAB |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000290234800013 |
| 源URL | [http://ir.wipm.ac.cn/handle/112942/1789]  |
| 专题 | 武汉物理与数学研究所_2011年以前论文发表（包括2011年） |
| 作者单位 | 1.Chinese Acad Sci, State Key Lab Magnet Resonance & Atom & Mol Phys, Wuhan Ctr Magnet Resonance, Wuhan Inst Phys & Math, Wuhan 430071, Peoples R China 2.Huazhong Agr Univ, Mol Biotechnol Lab Triticeae Crops, Wuhan 430070, Peoples R China 3.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China 4.Huazhong Agr Univ, Coll Plant Sci & Technol, Wuhan 430070, Peoples R China 5.Natl Ctr Plant Gene Res Wuhan, Wuhan 430070, Peoples R China |
| 推荐引用方式 GB/T 7714 | Chen, Fangfang,Zhang, Jingtao,Song, Xiushi,et al. Combined Metabonomic and Quantitative Real-Time PCR Analyses Reveal Systems Metabolic Changes of Fusarium graminearum Induced by Tri5 Gene Deletion[J]. JOURNAL OF PROTEOME RESEARCH,2011,10(5):2273-2285. |
| APA | Chen, Fangfang.,Zhang, Jingtao.,Song, Xiushi.,Yang, Jian.,Li, Heping.,...&Liao, Yu-Cai.(2011).Combined Metabonomic and Quantitative Real-Time PCR Analyses Reveal Systems Metabolic Changes of Fusarium graminearum Induced by Tri5 Gene Deletion.JOURNAL OF PROTEOME RESEARCH,10(5),2273-2285. |
| MLA | Chen, Fangfang,et al."Combined Metabonomic and Quantitative Real-Time PCR Analyses Reveal Systems Metabolic Changes of Fusarium graminearum Induced by Tri5 Gene Deletion".JOURNAL OF PROTEOME RESEARCH 10.5(2011):2273-2285. |
入库方式： OAI收割
来源：武汉物理与数学研究所
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