Proton NMR Based Investigation of the Effects of Temperature and NaCl on Micellar Properties of CHAPS
文献类型：期刊论文
| 作者 | Qin, Xianguo1,2; Liu, Maili1; Zhang, Xu1; Yang, Daiwen3 |
| 刊名 | JOURNAL OF PHYSICAL CHEMISTRY B
 |
| 出版日期 | 2011-03-10 |
| 卷号 | 115期号:9页码:1991-1998 |
| 产权排序 | 第一 |
| 通讯作者 | Yang,DW |
| 英文摘要 | The effects of temperature and NaCl on the micellization of CHAPS, a zwitterionic detergent widely used in membrane protein studies, have been investigated by NMR spectroscopy. We found that the two apparent critical micelle concentration (cmc) values of CHAPS decrease with the increase of temperature, as well as the NaCl concentration. The thermodynamic parameters derived from the temperature dependent cmc values show that the micellization process is spontaneous and exothermic, and the van der Waals interaction is likely to be the main factor for the micellization of CHAPS. The micellar hydrodynamic radii remain almost the same in a range of 100-600 mM NaCl, indicating that the aggregate states of CHAPS are not sensitive to the change of the surrounding conditions. In addition, the dependence of nuclear Overhauser effect (NOE) intensities on temperatures further demonstrates the existence of the unique staggered micellar structure of CHAPS at a concentration above the apparent second cmc, which was suggested in our previous work. Our results provide a basis for optimizing CHAPS concentration M the solubilization or stabilization of membrane proteins under nondenaturing conditions and may be helpful to understand its interaction with proteins. |
| WOS标题词 | Science & Technology ; Physical Sciences |
| 学科主题 | 波谱分析 |
| 类目[WOS] | Chemistry, Physical |
| 研究领域[WOS] | Chemistry |
| 关键词[WOS] | SODIUM DODECYL-SULFATE ; MEMBRANE-PROTEINS ; IONIC-STRENGTH ; ZWITTERIONIC DETERGENT ; AGGREGATION BEHAVIOR ; THERMOTOGA-MARITIMA ; TRIBLOCK COPOLYMER ; AQUEOUS-SOLUTIONS ; SPIN-DIFFUSION ; MICELLIZATION |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000287833000009 |
| 源URL | [http://ir.wipm.ac.cn/handle/112942/1810]  |
| 专题 | 武汉物理与数学研究所_2011年以前论文发表（包括2011年） |
| 作者单位 | 1.Chinese Acad Sci, Wuhan Inst Phys & Math, State Key Lab Magnet Resonance & Atom & Mol Phys, Wuhan Ctr Magnet Resonance, Wuhan 430071, Peoples R China 2.Huazhong Univ Sci & Technol, Sch Phys, Wuhan 430074, Peoples R China 3.Natl Univ Singapore, Dept Biol Sci, Singapore 117543, Singapore |
| 推荐引用方式 GB/T 7714 | Qin, Xianguo,Liu, Maili,Zhang, Xu,et al. Proton NMR Based Investigation of the Effects of Temperature and NaCl on Micellar Properties of CHAPS[J]. JOURNAL OF PHYSICAL CHEMISTRY B,2011,115(9):1991-1998. |
| APA | Qin, Xianguo,Liu, Maili,Zhang, Xu,&Yang, Daiwen.(2011).Proton NMR Based Investigation of the Effects of Temperature and NaCl on Micellar Properties of CHAPS.JOURNAL OF PHYSICAL CHEMISTRY B,115(9),1991-1998. |
| MLA | Qin, Xianguo,et al."Proton NMR Based Investigation of the Effects of Temperature and NaCl on Micellar Properties of CHAPS".JOURNAL OF PHYSICAL CHEMISTRY B 115.9(2011):1991-1998. |
入库方式： OAI收割
来源：武汉物理与数学研究所
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