Dynamic Metabolic Response of Mice to Acute Mequindox Exposure
文献类型：期刊论文
| 作者 | Zhao, Xiu-Ju1,2; Huang, Chongyang1,2; Lei, Hehua1; Nie, Xiu3; Tang, Huiru1; Wang, Yulan1 |
| 刊名 | JOURNAL OF PROTEOME RESEARCH
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| 出版日期 | 2011-11-01 |
| 卷号 | 10期号:11页码:5183-5190 |
| 关键词 | NMR pattern recognition metabonomics mequindox dose effect time effect mice |
| 产权排序 | 第一 |
| 英文摘要 | Mequindox is used as a veterinary antibiotic drug. As part of systematic investigations into mequindox as a veterinary medicine and its subsequent applications in food safety, we conducted the investigation to assess the metabolic response of mice to mequindox using metabonomics, which combines NMR metabolic profiles of biofluids or tissues and pattern recognition data analysis. In this study, we delivered a single dose of mequindox to mice with dosage levels of 15, 75, and 350 mg/kg body weight and collected urine samples over a 7 day period, as well as plasma and liver tissues at 7 days postdose. Principal components analysis (PCA) and orthogonal projection to latent structure discriminant analysis (O-PLS-DA) were performed on (1)H NMR spectra of biofluids and liver, showing that low dose levels of mequindox exposure had no adverse effects, consistent with histological observations of the liver. High and moderate levels of mequindox exposure caused suppression of glycolysis and stimulation of fatty acid oxidation accompanied with increased levels of oxidative stress. Our metabonomic analyses also showed disruption of amino acid metabolism, consistent with liver damage observed from histopathological examinations. Furthermore, mequindox perturbed gut microbial activity manifested in the altered excretion of urinary trimethylamine (TMA), trimethylamine-N-oxide (TMAO), hippurate, phenylacetylglycine (PAG), and phenylacetate. The putative gut microbial function may also contribute to the assembly and secretion of very-low-density lipoproteins from the liver to the plasma. Our work provides important insights on the metabolic responses of mequindox. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 学科主题 | 波谱学 |
| 类目[WOS] | Biochemical Research Methods |
| 研究领域[WOS] | Biochemistry & Molecular Biology |
| 关键词[WOS] | PRINCIPAL COMPONENT ANALYSIS ; MATRICARIA-RECUTITA L. ; LEAST-SQUARES METHODS ; METABONOMIC INVESTIGATIONS ; MAGNETIC-RESONANCE ; SPECTRAL ANALYSES ; MODEL ; RATS ; PLS ; CLASSIFICATION |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000296414700026 |
| 源URL | [http://ir.wipm.ac.cn/handle/112942/1892]  |
| 专题 | 武汉物理与数学研究所_2011年以前论文发表（包括2011年） |
| 作者单位 | 1.Chinese Acad Sci, Wuhan Ctr Magnet Resonance, State Key Lab Magnet Resonance & Atom & Mol Phys, Wuhan Inst Phys & Math, Wuhan 430071, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China 3.Huazhong Univ Sci & Technol, Dept Pathol, Union Hosp, Tongji Med Coll, Wuhan 430022, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhao, Xiu-Ju,Huang, Chongyang,Lei, Hehua,et al. Dynamic Metabolic Response of Mice to Acute Mequindox Exposure[J]. JOURNAL OF PROTEOME RESEARCH,2011,10(11):5183-5190. |
| APA | Zhao, Xiu-Ju,Huang, Chongyang,Lei, Hehua,Nie, Xiu,Tang, Huiru,&Wang, Yulan.(2011).Dynamic Metabolic Response of Mice to Acute Mequindox Exposure.JOURNAL OF PROTEOME RESEARCH,10(11),5183-5190. |
| MLA | Zhao, Xiu-Ju,et al."Dynamic Metabolic Response of Mice to Acute Mequindox Exposure".JOURNAL OF PROTEOME RESEARCH 10.11(2011):5183-5190. |
入库方式： OAI收割
来源：武汉物理与数学研究所
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