Nuclear Magnetic Resonance for Analysis of Metabolite Composition of Escherichia coli
文献类型：期刊论文
| 作者 | Ye Yang-Fang1,2; Zhang Li-Min1; An Yan-Peng1; Hao Fu-Hua1; Tang Hui-Ru1 |
| 刊名 | CHINESE JOURNAL OF ANALYTICAL CHEMISTRY
 |
| 出版日期 | 2011-08-01 |
| 卷号 | 39期号:8页码:1186-1194 |
| 关键词 | Escherichia coli Metabolome Nuclear magnetic resonance |
| 产权排序 | 第一 |
| 英文摘要 | To understand the composition of endogenous metabolites of Escherichia coli an important model organism, a series of high-resolution NMR spectroscopic methods were employed to analyze the metabolites of this strain qualitatively and quantitatively. We found that E. coli metabolome was dominated by more than 40 abundant metabolites including amino acids, organic acids, sugars, nucleoside and its derivatives involving many different metabolic pathways. We further found that glucose level was the highest in stationary-phase E. coli while glutamate, betaine, putrescine and ribose-5-phosphate levels were higher than other metabolites. Our results indicated that NMR method was effective to simultaneously detect the abundant bacterial metabolites of different polarity including both the primary and secondary metabolites. |
| 学科主题 | 波谱学 |
| WOS标题词 | Science & Technology ; Physical Sciences |
| 类目[WOS] | Chemistry, Analytical |
| 研究领域[WOS] | Chemistry |
| 关键词[WOS] | MASS-SPECTROMETRY ; METABOLOMICS ; NMR ; QUANTIFICATION ; SPECTROSCOPY ; EXTRACTION ; PHENOTYPE ; STRATEGY ; STRAINS |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000296599800012 |
| 源URL | [http://ir.wipm.ac.cn/handle/112942/1925]  |
| 专题 | 武汉物理与数学研究所_2011年以前论文发表（包括2011年） |
| 作者单位 | 1.Chinese Acad Sci, State Key Lab Magnet Resonance & Atom & Mol Phys, Wuhan Inst Phys & Math, Wuhan 430071, Peoples R China 2.Ningbo Univ, Key Lab Appl Marine Biotechnol, Minist Educ, Ningbo 315211, Zhejiang, Peoples R China |
| 推荐引用方式 GB/T 7714 | Ye Yang-Fang,Zhang Li-Min,An Yan-Peng,et al. Nuclear Magnetic Resonance for Analysis of Metabolite Composition of Escherichia coli[J]. CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,2011,39(8):1186-1194. |
| APA | Ye Yang-Fang,Zhang Li-Min,An Yan-Peng,Hao Fu-Hua,&Tang Hui-Ru.(2011).Nuclear Magnetic Resonance for Analysis of Metabolite Composition of Escherichia coli.CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,39(8),1186-1194. |
| MLA | Ye Yang-Fang,et al."Nuclear Magnetic Resonance for Analysis of Metabolite Composition of Escherichia coli".CHINESE JOURNAL OF ANALYTICAL CHEMISTRY 39.8(2011):1186-1194. |
入库方式： OAI收割
来源：武汉物理与数学研究所
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