Combined NMR and LC-MS Analysis Reveals the Metabonomic Changes in Salvia miltiorrhiza Bunge Induced by Water Depletion
文献类型：期刊论文
| 作者 | Dai, Hui1,3; Xiao, Chaoni1,2,3; Liu, Hongbing1,3; Tang, Huiru1 |
| 刊名 | JOURNAL OF PROTEOME RESEARCH
 |
| 出版日期 | 2010-03-01 |
| 卷号 | 9期号:3页码:1460-1475 |
| 关键词 | Salvia miltiorrhiza Bunge NMR LC-DAD-MS PCA (principal component analysis) OPLS-DA (orthogonal projection to latent structure with discriminant analysis) |
| 产权排序 | 第一 |
| 英文摘要 | Plant metabonomic analysis is essential for understanding plant systems responses to osmotic stresses. To understand the comprehensive metabolic responses of Salvia miltiorrhiza Bunge (SMB) to continuous and exhaustive water depletion, we characterized the SMB metabonomic variations induced by three different drying processes using the combined NMR and LC-DAD-MS method. NMR results showed that SMB extracts were dominated by 29 primary metabolites such as sugars, carboxylic acids and amino acids, which were comprehensively reported for the first time, and 8 secondary metabolites including polyphenolic acids and diterpenoids. LC-DAD-MS methods detected 44 secondary metabolites, among which 5 polyphenolic acids together with genipin, umbelliferone and tormentic acid were found for the first time in this plant. We found that aqueous methanol was efficient in extracting both primary metabolites and polyphenolic acids, whereas chloroform-methanol was effective in selectively extracting diterpenoids. We further found that air- and sun-drying markedly affected both primary and secondary metabolisms of SMB by enhancing tanshinone and glutamate-mediated proline biosynthesis and altering carbohydrate and amino acid metabolisms. The shikimate-mediated biosynthesis of polyphenolic acids was promoted by air-drying but suppressed by sun-drying. These findings fill the gap of our understandings to the metabolic responses of S. miltiorrhiza Bunge to water depletion and demonstrated effectiveness of the combined NMR and LC-DAD-MS methods in plant metabonomic analysis. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 学科主题 | 波谱分析 |
| 类目[WOS] | Biochemical Research Methods |
| 研究领域[WOS] | Biochemistry & Molecular Biology |
| 关键词[WOS] | NUCLEAR-MAGNETIC-RESONANCE ; MULTIVARIATE DATA-ANALYSIS ; DIFFERENT DRYING METHODS ; H-1-NMR SPECTROSCOPY ; ANTIOXIDANT PROPERTIES ; PROLINE BIOSYNTHESIS ; ARABIDOPSIS-THALIANA ; METABOLIC-RESPONSES ; FUNCTIONAL GENOMICS ; COMPLEX-MIXTURES |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000275088100027 |
| 源URL | [http://ir.wipm.ac.cn/handle/112942/1978]  |
| 专题 | 武汉物理与数学研究所_2011年以前论文发表（包括2011年） |
| 作者单位 | 1.Chinese Acad Sci, Wuhan Inst Phys & Math, Wuhan Ctr Magnet Resonance, State Key Lab Magnet Resonance & Atom & Mol Phys, Wuhan 430071, Peoples R China 2.NW Univ Xian, Coll Life Sci, Xian 710069, Peoples R China 3.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Dai, Hui,Xiao, Chaoni,Liu, Hongbing,et al. Combined NMR and LC-MS Analysis Reveals the Metabonomic Changes in Salvia miltiorrhiza Bunge Induced by Water Depletion[J]. JOURNAL OF PROTEOME RESEARCH,2010,9(3):1460-1475. |
| APA | Dai, Hui,Xiao, Chaoni,Liu, Hongbing,&Tang, Huiru.(2010).Combined NMR and LC-MS Analysis Reveals the Metabonomic Changes in Salvia miltiorrhiza Bunge Induced by Water Depletion.JOURNAL OF PROTEOME RESEARCH,9(3),1460-1475. |
| MLA | Dai, Hui,et al."Combined NMR and LC-MS Analysis Reveals the Metabonomic Changes in Salvia miltiorrhiza Bunge Induced by Water Depletion".JOURNAL OF PROTEOME RESEARCH 9.3(2010):1460-1475. |
入库方式： OAI收割
来源：武汉物理与数学研究所
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