Combined NMR and LC-DAD-MS Analysis Reveals Comprehensive Metabonomic Variations for Three Phenotypic Cultivars of Salvia Miltiorrhiza Bunge
文献类型：期刊论文
| 作者 | Dai, Hui1,3; Xiao, Chaoni1,2,3; Liu, Hongbing1,3; Hao, Fuhua1 ; Tang, Huiru1
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| 刊名 | JOURNAL OF PROTEOME RESEARCH
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| 出版日期 | 2010-03-01 |
| 卷号 | 9期号:3页码:1565-1578 |
| 关键词 | Salvia miltiorrhiza Bunge (SMB) NMR LC-DAD-MS ecotype PCA (principal component analysis) OPLS-DA (orthogonal projection to latent structure with discriminant analysis) |
| 产权排序 | 第一 |
| 英文摘要 | Metabonomic analysis is an important molecular phenotyping method for understanding plant ecotypic variations and gene functions. Here, we systematically characterized the metabonomic variations associated with three Salvia miltiorrhiza Bunge (SMB) cultivars using the combined NMR and LC-DAD-MS detections in conjunction with multivariate data analysis. Our results indicated that NMR methods were effective to quantitatively detect the abundant plant metabolites including both the primary and secondary metabolites whereas the LC-DAD-MS methods were excellent for selectively detecting the secondary metabolites. We found that the SMB metabonome was dominated by 28 primary metabolites including sugars, amino acids, and carboxylic acids and 4 polyphenolic secondary metabolites, among which N-acetylglutamate, asparate, fumurate, and yunnaneic acid D were reported for the first time in this plant. We also found that three SMB cultivars growing at the same location had significant metabonomic differences in terms of metabolisms of carbohydrates, amino acids, and choline, TCA cycle, and the shikimate-mediated secondary metabolisms. We further found that the same SMB cultivar growing at different locations differed in their metabonome. These results provided important information on the ecotypic dependence of SMB metabonome on the growing environment and demonstrated that the combination of NMR and LC-MS methods was effective for plant metabonomic phenotype analysis. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 学科主题 | 波谱分析 |
| 类目[WOS] | Biochemical Research Methods |
| 研究领域[WOS] | Biochemistry & Molecular Biology |
| 关键词[WOS] | MULTIVARIATE DATA-ANALYSIS ; H-1-NMR SPECTROSCOPY ; FUNCTIONAL GENOMICS ; LIQUID-CHROMATOGRAPHY ; METABOLIC-RESPONSES ; HEART-DISEASE ; GREEN TEA ; QUALITY ; SYSTEMS ; LEAVES |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000275088100036 |
| 源URL | [http://ir.wipm.ac.cn/handle/112942/1981]  |
| 专题 | 武汉物理与数学研究所_2011年以前论文发表（包括2011年） |
| 作者单位 | 1.Chinese Acad Sci, Wuhan Inst Phys & Math, Wuhan Ctr Magnet Resonance, State Key Lab Magnet Resonance & Atom & Mol Phys, Wuhan 430071, Peoples R China 2.NW Univ Xian, Coll Life Sci, Xian 710069, Peoples R China 3.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Dai, Hui,Xiao, Chaoni,Liu, Hongbing,et al. Combined NMR and LC-DAD-MS Analysis Reveals Comprehensive Metabonomic Variations for Three Phenotypic Cultivars of Salvia Miltiorrhiza Bunge[J]. JOURNAL OF PROTEOME RESEARCH,2010,9(3):1565-1578. |
| APA | Dai, Hui,Xiao, Chaoni,Liu, Hongbing,Hao, Fuhua,&Tang, Huiru.(2010).Combined NMR and LC-DAD-MS Analysis Reveals Comprehensive Metabonomic Variations for Three Phenotypic Cultivars of Salvia Miltiorrhiza Bunge.JOURNAL OF PROTEOME RESEARCH,9(3),1565-1578. |
| MLA | Dai, Hui,et al."Combined NMR and LC-DAD-MS Analysis Reveals Comprehensive Metabonomic Variations for Three Phenotypic Cultivars of Salvia Miltiorrhiza Bunge".JOURNAL OF PROTEOME RESEARCH 9.3(2010):1565-1578. |
入库方式： OAI收割
来源：武汉物理与数学研究所
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