An optimised sample preparation method for NMR-based faecal metabonomic analysis
文献类型：期刊论文
| 作者 | Wu, Junfang1,2; An, Yanpeng1,2; Yao, Jianwu1,2; Wang, Yulan1; Tang, Huiru1 |
| 刊名 | ANALYST
 |
| 出版日期 | 2010 |
| 卷号 | 135期号:5页码:1023-1030 |
| 产权排序 | 第一 |
| 英文摘要 | Faecal metabonomic NMR analysis plays an essential role in investigating the interactions between mammalian metabolism and symbiotic gut microbiota. However, the faecal metabolite extraction method remains to be optimised and standardised to take into consideration signal-to-noise ratios, pH and chemical shift consistency. In the current investigation, we compared extraction consistency of three homogenisation methods including manual ultrasonication, automatic homogenization with tissuelyser and their combination, and systematically optimised faecal metabolite extraction parameters, including the faeces-to-buffer ratio (W(f) : V(b)), extraction repetition times and duration. We found that automatic homogenisation with tissuelyser was the choice of extraction method owning to its good metabolite extraction consistency and high throughput. We also recommend W(f) : V(b) of 1 : 10 (mg mu l(-1)) and use of the combined first two extracts as the resultant samples to represent faecal metabolite composition. Such recommendation is based on considerations of maximisation of the spectral signal-to-noise ratio, pH and chemical shift consistency, completeness of metabolite extraction and sample preparation throughput so that the method is suitable for analysing a large number of samples especially in human population studies. |
| WOS标题词 | Science & Technology ; Physical Sciences |
| 学科主题 | 波谱分析 |
| 类目[WOS] | Chemistry, Analytical |
| 研究领域[WOS] | Chemistry |
| 关键词[WOS] | METABOLIC INTERACTIONS ; GUT MICROBES ; MOUSE MODEL ; BIOMARKER ; IDENTIFICATION ; INFECTION ; RESPONSES ; PROFILES ; URINE ; WATER |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000277037500025 |
| 源URL | [http://ir.wipm.ac.cn/handle/112942/1984]  |
| 专题 | 武汉物理与数学研究所_2011年以前论文发表（包括2011年） |
| 作者单位 | 1.Chinese Acad Sci, State Key Lab Magnet Resonance & Atom & Mol Phys, Wuhan Ctr Magnet Resonance, Wuhan Inst Phys & Math, Wuhan 430071, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wu, Junfang,An, Yanpeng,Yao, Jianwu,et al. An optimised sample preparation method for NMR-based faecal metabonomic analysis[J]. ANALYST,2010,135(5):1023-1030. |
| APA | Wu, Junfang,An, Yanpeng,Yao, Jianwu,Wang, Yulan,&Tang, Huiru.(2010).An optimised sample preparation method for NMR-based faecal metabonomic analysis.ANALYST,135(5),1023-1030. |
| MLA | Wu, Junfang,et al."An optimised sample preparation method for NMR-based faecal metabonomic analysis".ANALYST 135.5(2010):1023-1030. |
入库方式： OAI收割
来源：武汉物理与数学研究所
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