F-19 NMR studies of alpha-synuclein-membrane interactions
文献类型：期刊论文
| 作者 | Wang, Gui-Fang; Li, Conggang2; Pielak, Gary J.1 |
| 刊名 | PROTEIN SCIENCE
 |
| 出版日期 | 2010-09-01 |
| 卷号 | 19期号:9页码:1686-1691 |
| 关键词 | binding F-19 NMR membranes alpha-synuclein |
| 产权排序 | 第二 |
| 英文摘要 | alpha-Synuclein function is thought to be related to its membrane binding ability. Solution NMR studies have identified several alpha-synuclein-membrane interaction modes in small unilamellar vesicles (SUVs), but how membrane properties affect binding remains unclear. Here, we use F-19 NMR to study alpha-synuclein-membrane interactions by using 3-fluoro-L-tyrosine (3FY) and trifluoromethyl-L-phenylalanine (tfmF) labeled proteins. Our results indicate that the affinity is affected by both the head group and the acyl chain of the SUV. Negatively charged head groups have higher affinity, but different head groups with the same charge also affect binding. We show that the saturation of the acyl chain has a dramatic effect on the alpha-synuclein-membrane interactions by studying lipids with the same head group but different chains. Taken together, the data show that alpha-synuclein's N-terminal region is the most important determinate of SUV binding, but its C-terminal region also modulates the interactions. Our data support the existence of multiple tight phospholipid-binding modes, a result incompatible with the model that alpha-synuclein lies solely on the membrane surface. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 学科主题 | 波谱分析 |
| 类目[WOS] | Biochemistry & Molecular Biology |
| 研究领域[WOS] | Biochemistry & Molecular Biology |
| 关键词[WOS] | DISEASE-LINKED MUTATIONS ; PARKINSONS-DISEASE ; PHOSPHOLIPID-BINDING ; ALZHEIMERS-DISEASE ; PROTEIN-STRUCTURE ; SPECTROSCOPY ; VESICLES ; HELIX ; FLUORESCENCE ; ASSOCIATION |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000281563000008 |
| 源URL | [http://ir.wipm.ac.cn/handle/112942/2036]  |
| 专题 | 武汉物理与数学研究所_2011年以前论文发表（包括2011年） |
| 作者单位 | 1.Univ N Carolina, Dept Chem, Dept Biochem & Biophys, Lineberger Comprehens Canc Ctr, Chapel Hill, NC 27599 USA 2.Chinese Acad Sci, Wuhan Inst Phys & Math, State Key Lab Magnet Resonance & Mol & Atom Phys, Wuhan 430071, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Gui-Fang,Li, Conggang,Pielak, Gary J.. F-19 NMR studies of alpha-synuclein-membrane interactions[J]. PROTEIN SCIENCE,2010,19(9):1686-1691. |
| APA | Wang, Gui-Fang,Li, Conggang,&Pielak, Gary J..(2010).F-19 NMR studies of alpha-synuclein-membrane interactions.PROTEIN SCIENCE,19(9),1686-1691. |
| MLA | Wang, Gui-Fang,et al."F-19 NMR studies of alpha-synuclein-membrane interactions".PROTEIN SCIENCE 19.9(2010):1686-1691. |
入库方式： OAI收割
来源：武汉物理与数学研究所
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