Tuftsin derivatives of FITC, Tb-DOTA or Gd-DOTA as potential macrophage-specific imaging biomarkers
文献类型：期刊论文
| 作者 | Feng, Jianghua1,2; Meloni, Marco M.3; Allan, Stuart M.2; Faulkner, Stephen4; Narvainen, Johanna5; Vidyasagar, Rishma2; Kauppinen, Risto6 |
| 刊名 | CONTRAST MEDIA & MOLECULAR IMAGING
 |
| 出版日期 | 2010-07-01 |
| 卷号 | 5期号:4页码:223-230 |
| 关键词 | macrophage magnetic resonance imaging contrast agent tuftsin |
| 产权排序 | 第一 |
| 英文摘要 | Fluorescein- and terbium-labelled tuftsin (Thr-Lys-Pro-Arg) and pentapeptide (Thr-Lys-Pro-Pro-Arg) were synthesized and their properties were evaluated in vitro by luminescence spectrometry and confocal microscopy as fluorescence probes to target macrophage cells in biological systems. An increase in fluorescence of macrophages incubated with varying concentrations of fluorescein isothiocyanate or Tb-DOTA-tuftsin/pentapeptide conjugates was observed in a concentration-dependent manner. Tb-DOTA-pentapeptide had a greater affinity to macrophages than Tb-DOTA-tuftsin. Lipopolysaccharide (LPS) stimulation strengthened the internalization of peptide conjugates by macrophages through the tuftsin receptor mechanism. Tb-DOTA-tuftsin/pentapeptide conjugates are likely to be a(dagger) promising optical reagents as probes of the immune response with involvement of macrophage cells in a variety of diseases. Gd-DOTA-tuftsin conjugate was also evaluated as a cell-specific contrast agent in in vitro MRI experiments. In this context, the macrophages labelled by Gd-DOTA-tuftsin were highly magnetic and detectable by MRI, which confirms that this vectorized MRI probe has the potential to image macrophage-mediated inflammation in diseases like brain traumas and stroke. Tuftsin receptor-specific biological-function domain may have a modified in vivo biodistribution profile, bioavailability and pharmacokinetics subsequent to its conjugation to a metal ion-binding backbone. Copyright (C) 2010 John Wiley & Sons, Ltd. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 学科主题 | 波谱分析 |
| 类目[WOS] | Radiology, Nuclear Medicine & Medical Imaging |
| 研究领域[WOS] | Radiology, Nuclear Medicine & Medical Imaging |
| 关键词[WOS] | MRI CONTRAST AGENTS ; INTRACEREBRAL HEMORRHAGE ; INFLAMMATORY CYTOKINES ; CEREBRAL-ISCHEMIA ; BEARING LIPOSOMES ; BRAIN-INJURY ; FRAGMENT 1-3 ; CELL-LINE ; IN-VIVO ; ACTIVATION |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000281693500007 |
| 源URL | [http://ir.wipm.ac.cn/handle/112942/2037]  |
| 专题 | 武汉物理与数学研究所_2011年以前论文发表（包括2011年） |
| 作者单位 | 1.Chinese Acad Sci, Wuhan Inst Phys & Math, State Key Lab Magnet Resonance & Mol & Atom Phys, Wuhan 430071, Peoples R China 2.Univ Manchester, Fac Life Sci, Manchester M13 9PT, Lancs, England 3.Univ Manchester, Sch Chem, Manchester M13 9PT, Lancs, England 4.Univ Oxford, Dept Chem, Oxford OX1 3TA, England 5.Univ Eastern Finland, AI Virtanen Inst, Kuopio, Finland 6.Dartmouth Coll, Dept Radiol, Hanover, NH 03755 USA |
| 推荐引用方式 GB/T 7714 | Feng, Jianghua,Meloni, Marco M.,Allan, Stuart M.,et al. Tuftsin derivatives of FITC, Tb-DOTA or Gd-DOTA as potential macrophage-specific imaging biomarkers[J]. CONTRAST MEDIA & MOLECULAR IMAGING,2010,5(4):223-230. |
| APA | Feng, Jianghua.,Meloni, Marco M..,Allan, Stuart M..,Faulkner, Stephen.,Narvainen, Johanna.,...&Kauppinen, Risto.(2010).Tuftsin derivatives of FITC, Tb-DOTA or Gd-DOTA as potential macrophage-specific imaging biomarkers.CONTRAST MEDIA & MOLECULAR IMAGING,5(4),223-230. |
| MLA | Feng, Jianghua,et al."Tuftsin derivatives of FITC, Tb-DOTA or Gd-DOTA as potential macrophage-specific imaging biomarkers".CONTRAST MEDIA & MOLECULAR IMAGING 5.4(2010):223-230. |
入库方式： OAI收割
来源：武汉物理与数学研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。