H-1-N-14 HSQC detection of choline-containing compounds in solutions
文献类型：期刊论文
| 作者 | Mao, Jiezhen1; Jiang, Ling1; Jiang, Bin1 ; Liu, Maili1; Mao, Xi-an1,2
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| 刊名 | JOURNAL OF MAGNETIC RESONANCE
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| 出版日期 | 2010-09-01 |
| 卷号 | 206期号:1页码:157-160 |
| 关键词 | H-1-N-14 HSQC Choline Solution Milk |
| 产权排序 | 第一 |
| 英文摘要 | Choline nitrogen (N-14) has a long relaxation time (seconds) which is due to the highly symmetric chemical environments. N-14 in choline also has coupling constants with protons (0.6 Hz to methyl protons. 2.7 Hz to CH2O protons and 0.2 Hz to NCH2 protons). Based on these properties, we introduce a two-dimensional NMR method to detect choline and its derivatives in solutions. This method is the H-1-N-14 hetero-nuclear single-quantum correlation (HSQC) experiment which has been developed in solid-state NMR in recent years. Experiments have demonstrated that the H-1-N-14 HSQC technique is a sensitive method for detection of choline-containing compounds in solutions. From 1 mM choline solution in 16 min on a 500 MHz NMR spectrometer, a H-1-N-14 HSQC spectrum has been recorded with a signal-to-noise ratio of 1700. Free choline, phosphocholine and glycerophosphocholine in milk can be well separated in H-1-N-14 HSQC spectra. This technique would become a promising analytical approach to mixture analyses where choline-containing compounds are of interest, such as tissue extracts, body fluids and food solutions. (C) 2010 Elsevier Inc. All rights reserved. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences ; Technology |
| 学科主题 | 波谱分析 |
| 类目[WOS] | Biochemical Research Methods ; Physics, Atomic, Molecular & Chemical ; Spectroscopy |
| 研究领域[WOS] | Biochemistry & Molecular Biology ; Physics ; Spectroscopy |
| 关键词[WOS] | MAGNETIC-RESONANCE-SPECTROSCOPY ; N-14 NMR-SPECTROSCOPY ; ROTARY RESONANCE ; H-1 ; METABOLITES ; SOLIDS ; CANCER |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000281507600022 |
| 源URL | [http://ir.wipm.ac.cn/handle/112942/2039]  |
| 专题 | 武汉物理与数学研究所_2011年以前论文发表（包括2011年） |
| 作者单位 | 1.Chinese Acad Sci, Wuhan Inst Phys & Math, State Key Lab Magnet Resonance & Atom & Mol Phys, Wuhan 430071, Peoples R China 2.Case Western Reserve Univ, Sch Med, Dept Pharmacol, Cleveland, OH 44106 USA |
| 推荐引用方式 GB/T 7714 | Mao, Jiezhen,Jiang, Ling,Jiang, Bin,et al. H-1-N-14 HSQC detection of choline-containing compounds in solutions[J]. JOURNAL OF MAGNETIC RESONANCE,2010,206(1):157-160. |
| APA | Mao, Jiezhen,Jiang, Ling,Jiang, Bin,Liu, Maili,&Mao, Xi-an.(2010).H-1-N-14 HSQC detection of choline-containing compounds in solutions.JOURNAL OF MAGNETIC RESONANCE,206(1),157-160. |
| MLA | Mao, Jiezhen,et al."H-1-N-14 HSQC detection of choline-containing compounds in solutions".JOURNAL OF MAGNETIC RESONANCE 206.1(2010):157-160. |
入库方式： OAI收割
来源：武汉物理与数学研究所
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