Characterization of catabolic meta-nitrophenol nitroreductase from Cupriavidus necator JMP134
文献类型：期刊论文
| 作者 | Yin, Ying1; Xiao, Yi1; Liu, Hai-Zhou1; Hao, Fuhua2 ; Rayner, Simon1; Tang, Huiru2; Zhou, Ning-Yi1
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| 刊名 | APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
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| 出版日期 | 2010-08-01 |
| 卷号 | 87期号:6页码:2077-2085 |
| 关键词 | Catabolism Cupriavidus necator JMP134 meta-Nitrophenol Nitroreductase |
| 产权排序 | 第一 |
| 英文摘要 | Cupriavidus necator JMP134 utilizes meta-nitrophenol (MNP) as a sole source of carbon, nitrogen, and energy. The metabolic reconstruction of MNP degradation performed in silico suggested that the mnp cluster might have played important roles in MNP degradation. In order to experimentally confirm the prediction, we have now characterized mnpA-encoded meta-nitrophenol nitroreductase involved in the initial reaction of MNP degradation. Real-time PCR analysis indicated that mnpA played an essential role in MNP degradation. MnpA was purified to homogeneity as His-tagged proteins and was considered to be a dimer as determined by gel filtration. MnpA was an MNP nitroreductase with a tightly bound flavin mononucleotide (FMN), catalyzing the partial reduction of MNP to meta-hydroxylaminophenol via meta-nitrosophenol in the presence of NADPH and oxygen. The accumulation of meta-nitrosophenol was confirmed with the results of liquid chromatography-diode array detection and time-of-flight mass spectrometry for the first time. The low K (m) and high k (cat) of MnpA as well as MNP-inducible transcription of mnpA suggested that MNP was the physiological substrate for this nitroreductase. In addition, the phylogenetic analysis revealed that nitroreductases of known physiological function including MnpA constituted a new clade in the nitro-FMN-reductase superfamily. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 学科主题 | 波谱分析 |
| 类目[WOS] | Biotechnology & Applied Microbiology |
| 研究领域[WOS] | Biotechnology & Applied Microbiology |
| 关键词[WOS] | RALSTONIA-EUTROPHA JMP134 ; OXYGEN-INSENSITIVE NITROREDUCTASES ; PSEUDOMONAS-PUTIDA ; ESCHERICHIA-COLI ; AROMATIC-COMPOUNDS ; ENCODING ENZYMES ; P-NITROPHENOL ; GENE-CLUSTER ; DEGRADATION ; REDUCTION |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000280373100010 |
| 源URL | [http://ir.wipm.ac.cn/handle/112942/2046]  |
| 专题 | 武汉物理与数学研究所_2011年以前论文发表（包括2011年） |
| 作者单位 | 1.Chinese Acad Sci, Wuhan Inst Virol, State Key Lab Virol, Wuhan 430071, Peoples R China 2.Chinese Acad Sci, Wuhan Inst Phys & Math, Wuhan Ctr Magnet Resonance, State Key Lab Magnet Resonance & Atom & Mol Phys, Wuhan 430071, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yin, Ying,Xiao, Yi,Liu, Hai-Zhou,et al. Characterization of catabolic meta-nitrophenol nitroreductase from Cupriavidus necator JMP134[J]. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,2010,87(6):2077-2085. |
| APA | Yin, Ying.,Xiao, Yi.,Liu, Hai-Zhou.,Hao, Fuhua.,Rayner, Simon.,...&Zhou, Ning-Yi.(2010).Characterization of catabolic meta-nitrophenol nitroreductase from Cupriavidus necator JMP134.APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,87(6),2077-2085. |
| MLA | Yin, Ying,et al."Characterization of catabolic meta-nitrophenol nitroreductase from Cupriavidus necator JMP134".APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 87.6(2010):2077-2085. |
入库方式： OAI收割
来源：武汉物理与数学研究所
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