Human Urinary Metabolite Quantification in Nuclear Magnetic Resonance-based Metabonomics Using Electronic Internal Reference
文献类型：期刊论文
| 作者 | Yang Liang1,2; Ru Ge-Ying1,2; Tang Hui-Ru1; Liu Chao-Yang1 |
| 刊名 | CHINESE JOURNAL OF ANALYTICAL CHEMISTRY
 |
| 出版日期 | 2010-06-01 |
| 卷号 | 38期号:6页码:789-794 |
| 关键词 | Nuclear magnetic resonance Electronic internal reference Human urine Quantitative analysis |
| 产权排序 | 第一 |
| 英文摘要 | We report a complete assessment for urinary quantitative analysis by using an electronic signal as an internal reference, which was generated via decoupling channel on a Varian NMR spectrometer with its amplitude, frequency and transverse relaxation time digitally programmable according to a decoupling shape file. The electronic signal with excellent stability (standard deviation 0.4%) avoided possible problems such as insolubility, interaction and spectral overlap for a traditional internal reference. Concentration determination using the electronic signal proved to be reliable by a series of quantitative experiments. The relative error of quantification was about 1% and 5% when the analyte concentration was above 20 mmol/L and below 2 mmol/L respectively. We further measured the concentration of human urinary metabolites in a simultaneous fashion using this electronic internal reference and obtained consistent results with the values measured by the biochemical analysis instrument used in hospitals. |
| WOS标题词 | Science & Technology ; Physical Sciences |
| 学科主题 | 波谱分析 |
| 类目[WOS] | Chemistry, Analytical |
| 研究领域[WOS] | Chemistry |
| 关键词[WOS] | SOLID-STATE NMR ; QUANTITATIVE NMR ; ERETIC METHOD ; H-1-NMR SPECTROSCOPY ; HIGH-RESOLUTION ; HUMAN SERUM ; SIGNAL ; RELAXATION ; PROFILES ; PROBE |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000279604300005 |
| 源URL | [http://ir.wipm.ac.cn/handle/112942/2053]  |
| 专题 | 武汉物理与数学研究所_2011年以前论文发表（包括2011年） |
| 作者单位 | 1.Chinese Acad Sci, Wuhan Inst Phys & Math, State Key Lab Magnet Resonance & Atom & Mol Phys, Wuhan 430071, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100029, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yang Liang,Ru Ge-Ying,Tang Hui-Ru,et al. Human Urinary Metabolite Quantification in Nuclear Magnetic Resonance-based Metabonomics Using Electronic Internal Reference[J]. CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,2010,38(6):789-794. |
| APA | Yang Liang,Ru Ge-Ying,Tang Hui-Ru,&Liu Chao-Yang.(2010).Human Urinary Metabolite Quantification in Nuclear Magnetic Resonance-based Metabonomics Using Electronic Internal Reference.CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,38(6),789-794. |
| MLA | Yang Liang,et al."Human Urinary Metabolite Quantification in Nuclear Magnetic Resonance-based Metabonomics Using Electronic Internal Reference".CHINESE JOURNAL OF ANALYTICAL CHEMISTRY 38.6(2010):789-794. |
入库方式： OAI收割
来源：武汉物理与数学研究所
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