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High-resolution magic-angle-spinning NMR spectroscopy for metabolic profiling of intact tissues
文献类型：期刊论文
| 作者 | Beckonert, Olaf1; Coen, Muireann1; Keun, Hector C.1; Wang, Yulan2; Ebbels, Timothy M. D.1; Holmes, Elaine1; Lindon, John C.1; Nicholson, Jeremy K.1 |
| 刊名 | NATURE PROTOCOLS
 |
| 出版日期 | 2010 |
| 卷号 | 5期号:6页码:1019-1032 |
| 产权排序 | 第二 |
| 英文摘要 | Metabolic profiling, metabolomic and metabonomic studies require robust study protocols for any large-scale comparisons and evaluations. Detailed methods for solution-state NMR spectroscopy have been summarized in an earlier protocol. this protocol details the analysis of intact tissue samples by means of high-resolution magic-angle-spinning (HR-MAS) NMR spectroscopy and we provide a detailed description of sample collection, preparation and analysis. Described here are (1)H NMR spectroscopic techniques such as the standard one-dimensional, relaxation-edited, diffusion-edited and two-dimensional J-resolved pulse experiments, as well as one-dimensional (31)p NMR spectroscopy. these are used to monitor different groups of metabolites, e. g., sugars, amino acids and osmolytes as well as larger molecules such as lipids, non-invasively. Through the use of NMR-based diffusion coefficient and relaxation times measurements, information on molecular compartmentation and mobility can be gleaned. the NMR methods are often combined with statistical analysis for further metabonomics analysis and biomarker identification. the standard acquisition time per sample is 8-10 min for a simple one-dimensional (1)H NMR spectrum, giving access to metabolite information while retaining tissue integrity and hence allowing direct comparison with histopathology and MRI/MRS findings or the evaluation together with biofluid metabolic-profiling data. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 学科主题 | 波谱分析 |
| 类目[WOS] | Biochemical Research Methods |
| 研究领域[WOS] | Biochemistry & Molecular Biology |
| 关键词[WOS] | MAGNETIC-RESONANCE-SPECTROSCOPY ; HR-MAS SPECTROSCOPY ; PULSED-FIELD GRADIENTS ; H-1-NMR SPECTROSCOPY ; PATTERN-RECOGNITION ; BIOPSY SAMPLES ; IN-VIVO ; ALPHA-NAPHTHYLISOTHIOCYANATE ; TEMPERATURE CALIBRATION ; INDUCED HEPATOTOXICITY |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000278354700005 |
| 源URL | [http://ir.wipm.ac.cn/handle/112942/2058]  |
| 专题 | 武汉物理与数学研究所_2011年以前论文发表（包括2011年） |
| 作者单位 | 1.Univ London Imperial Coll Sci Technol & Med, Fac Med, Dept Surg & Canc, London, England 2.Chinese Acad Sci, Wuhan Inst Phys & Math, Wuhan Ctr Magnet Resonance, State Key Lab Magnet Resonance & Atom & Mol Phys, Wuhan, Peoples R China |
| 推荐引用方式 GB/T 7714 | Beckonert, Olaf,Coen, Muireann,Keun, Hector C.,et al. High-resolution magic-angle-spinning NMR spectroscopy for metabolic profiling of intact tissues[J]. NATURE PROTOCOLS,2010,5(6):1019-1032. |
| APA | Beckonert, Olaf.,Coen, Muireann.,Keun, Hector C..,Wang, Yulan.,Ebbels, Timothy M. D..,...&Nicholson, Jeremy K..(2010).High-resolution magic-angle-spinning NMR spectroscopy for metabolic profiling of intact tissues.NATURE PROTOCOLS,5(6),1019-1032. |
| MLA | Beckonert, Olaf,et al."High-resolution magic-angle-spinning NMR spectroscopy for metabolic profiling of intact tissues".NATURE PROTOCOLS 5.6(2010):1019-1032. |
入库方式： OAI收割
来源：武汉物理与数学研究所
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